DiPel-Selected Ostrinia nubilalis Larvae Are Not Resistant to Transgenic Corn Expressing Bacillus thuringiensis Cry1Ab

dc.contributor.author Li, Huarong
dc.contributor.author Bonning, Bryony
dc.contributor.author Buschman, Lawrent
dc.contributor.author Huang, Fangneng
dc.contributor.author Bonning, Bryony
dc.contributor.author Oppert, Brenda
dc.contributor.department Entomology
dc.date 2018-02-13T12:11:31.000
dc.date.accessioned 2020-06-30T02:25:26Z
dc.date.available 2020-06-30T02:25:26Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.embargo 2013-06-21
dc.date.issued 2007-01-01
dc.description.abstract <p>The survival of KS-SC DiPel-resistant and -susceptible European corn borer, <em>Ostrinia nubilalis</em> (Hübner), was evaluated on different tissues from corn, <em>Zea mays</em> L., hybrids, including a nontransgenic and two transgenic corn plants (events MON810 and Bt11) expressing high doses of <em>Bacillus thuringiensis</em> (Bt) Cry1Ab. The survival of Bt-resistant and -susceptible third instars was similar after a 5-d exposure to transgenic plant tissues. Survivors eventually died when returned to Bt corn tissues, but many were able to continue development when transferred to non-Bt corn tissues. Survival of resistant and susceptible larvae also was evaluated in bioassays with dilutions of leaf extracts from the three corn hybrids incorporated in an artificial diet. In these assays, survival was significantly higher for resistant <em>O. nubilalis</em> neonates at three of the five dilutions compared with the susceptible strain, but the resistance ratio was only 2.2- and 2.4-fold for MON810 and Bt11, respectively. The data demonstrate that Bt-resistant and unselected control <em>O. nubilalis</em> larvae were similar in susceptibility to MON810 and Bt11 event corn hybrids. Although we were unable to evaluate the Cry1Ab protein that larvae were exposed to in the transgenic tissue because of company restrictions, Cry1Ab protoxin produced in <em>Escherichia coli</em> was incubated with extracts from non-Bt corn leaves to simulate the in planta effect on the transgenic protein. Cry1Ab protoxin was hydrolyzed rapidly by enzymes in the corn extract into peptide fragments with molecular masses ranging from 132 to 74 kDa, and eventually 58 kDa. Overall, these data suggest that plant enzymes hydrolyze transgenic toxin to one that is functionally activated. Therefore, resistant insect populations with reduced proteinase activity do not seem to pose a threat to the efficacy of commercial MON810 and Bt11 corn hybrids.</p>
dc.description.comments <p>This article is from <em>Journal of Economic Entomology</em> 100, no. 6 (2007): 1862–1870, doi:<a href="http://dx.doi.org/10.1603/0022-0493(2007)100[1862:DONLAN]2.0.CO;2" target="_blank">10.1603/0022-0493(2007)100[1862:DONLAN]2.0.CO;2</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ent_pubs/7/
dc.identifier.articleid 1004
dc.identifier.contextkey 4250211
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/7
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/24228
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/7/2007_LiH_DiPelSelected.pdf|||Sat Jan 15 01:31:58 UTC 2022
dc.source.uri 10.1603/0022-0493(2007)100[1862:DONLAN]2.0.CO;2
dc.subject.disciplines Entomology
dc.subject.keywords Ostrinia nubilalis
dc.subject.keywords Bacillus thuringiensis
dc.subject.keywords resistance mechanisms
dc.subject.keywords resistance management
dc.subject.keywords proteinase-mediated resistance
dc.title DiPel-Selected Ostrinia nubilalis Larvae Are Not Resistant to Transgenic Corn Expressing Bacillus thuringiensis Cry1Ab
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication fa83bafa-bd16-4143-a293-61d8f7285f73
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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