Using spontaneous haploid genome doubling to access favorable alleles in exotic maize (Zea mays L.) germplasm

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Verzegnazzi, Anderson
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Thomas Lübberstedt
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The Department of Agronomy seeks to teach the study of the farm-field, its crops, and its science and management. It originally consisted of three sub-departments to do this: Soils, Farm-Crops, and Agricultural Engineering (which became its own department in 1907). Today, the department teaches crop sciences and breeding, soil sciences, meteorology, agroecology, and biotechnology.

The Department of Agronomy was formed in 1902. From 1917 to 1935 it was known as the Department of Farm Crops and Soils.

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  • Department of Farm Crops and Soils (1917–1935)

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Maize (Zea mays L.) breeding contributed to significant yield gains in the past decades. However, it has been accompanied by narrowing its germplasm base. Breeding from exotic maize germplasm, such as BS39, provides a unique opportunity for broadening the genetic base of US Corn Belt germplasm. The in vivo doubled haploid (DH) technology is widely used into exotic germplasms because it has a great promise in purging mutational load of deleterious recessive alleles. Accounting for all the improvements of DH production over the years, genome duplication is still a major bottleneck. Its efficiency tends to be reduced when the technology is applied in exotic germplasm. The use of spontaneous haploid genome doubling (SHGD) can improve DH production, including in exotic germplasm. In this study, 663 maize inbred lines were derived from exotic germplasm, BS39, by DH and single-seed descent (SSD) breeding methods. Genotype-by-Sequencing (GBS) and Diversity Array Technology Sequencing (DArtSeq) was used for genotyping to understand the impact of SHGD on exploiting exotic germplasm. Also, in a testcross combination we measured traits as grain yield, moisture, plant and ear height, and stalk and root lodging. Inbred lines derived from BS39 represent a novel source of genetic diversity for US Corn Belt germplasm. Results indicate that the presence of alleles conferring SHGD in a breeding population will not affect the selection of higher performance lines and enable efficient evaluation of gametes from breeding populations. However, when using SHGD the chromosome 5 region close to the centromere was enriched by the SHGD donor genome, probably caused by an allele affecting SHGD.

Fri May 01 00:00:00 UTC 2020