Host-Induced Gene Silencing in Barley Powdery Mildew Reveals a Class of Ribonuclease-Like Effectors

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dc.contributor.author Nettleton, Dan
dc.contributor.author Pliego, Clara
dc.contributor.author Nowara, Daniele
dc.contributor.author Surana, Priyanka
dc.contributor.author Bonciani, Giulia
dc.contributor.author Gheroghe, Dana
dc.contributor.author Xu, Ruo
dc.contributor.author Surana, Priyanka
dc.contributor.author Whigham, Ehren
dc.contributor.author Nettleton, Dan
dc.contributor.author Bogdanove, Adam
dc.contributor.author Wise, Roger
dc.contributor.author Schweizer, Patrick
dc.contributor.author Bindschedler, Laurence
dc.contributor.author Spanu, Pietro
dc.contributor.department Plant Pathology and Microbiology
dc.date 2018-02-16T11:35:31.000
dc.date.accessioned 2020-06-30T06:23:32Z
dc.date.available 2020-06-30T06:23:32Z
dc.date.issued 2013-06-01
dc.description.abstract <p>Obligate biotrophic pathogens of plants must circumvent or counteract defenses to guarantee accommodation inside the host. To do so, they secrete a variety of effectors that regulate host immunity and facilitate the establishment of pathogen feeding structures called haustoria. The barley powdery mildew fungus <em>Blumeria graminis</em> f. sp. <em>hordei</em>produces a large number of proteins predicted to be secreted from haustoria. Fifty of these <em>Blumeria</em> effector candidates (BEC) were screened by host-induced gene silencing (HIGS), and eight were identified that contribute to infection. One shows similarity to β-1,3 glucosyltransferases, one to metallo-proteases, and two to microbial secreted ribonucleases; the remainder have no similarity to proteins of known function. Transcript abundance of all eight BEC increases dramatically in the early stages of infection and establishment of haustoria, consistent with a role in that process. Complementation analysis using silencing-insensitive synthetic cDNAs demonstrated that the ribonuclease-like BEC 1011 and 1054 are bona fide effectors that function within the plant cell. BEC1011 specifically interferes with pathogen-induced host cell death. Both are part of a gene superfamily unique to the powdery mildew fungi. Structural modeling was consistent, with BEC1054 adopting a ribonuclease-like fold, a scaffold not previously associated with effector function.</p>
dc.description.comments <p>This article is from <em>Molecular Plant-Microbe Interactions</em> 26 (2013): 633, doi:<a href="http://dx.doi.org/10.1094/MPMI-01-13-0005-R" target="_blank">10.1094/MPMI-01-13-0005-R</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/29/
dc.identifier.articleid 1029
dc.identifier.contextkey 7183996
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/29
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57743
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/29/2013_Surana_HostInduced.pdf|||Fri Jan 14 23:13:11 UTC 2022
dc.source.uri 10.1094/MPMI-01-13-0005-R
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Pathology
dc.subject.keywords ascomycota
dc.subject.keywords cell death
dc.subject.keywords fungal proteins
dc.subject.keywords gene expression
dc.subject.keywords hordeum
dc.subject.keywords ribonucleases
dc.title Host-Induced Gene Silencing in Barley Powdery Mildew Reveals a Class of Ribonuclease-Like Effectors
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 7d86677d-f28f-4ab1-8cf7-70378992f75b
relation.isAuthorOfPublication d245c30f-4aeb-4750-a701-b676357ddaf8
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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