Effect of Surface Functionalization of MCM-41-Type Mesoporous Silica Nanoparticles on the Endocytosis by Human Cancer Cells

dc.contributor.author Slowing, Igor
dc.contributor.author Trewyn, Brian
dc.contributor.author Lin, Victor
dc.contributor.department Chemistry
dc.date 2018-02-17T06:43:14.000
dc.date.accessioned 2020-06-30T01:17:48Z
dc.date.available 2020-06-30T01:17:48Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.issued 2006-11-01
dc.description.abstract <p>We have synthesized a series of MCM-41-type mesoporous silica nanoparticles (MSN). The surface of the MSNs are functionalized with 3-aminopropyl (AP), 3-guanidinopropyl (GP), 3-[<em>N</em>-(2-guanidinoethyl)guanidino]propyl (GEGP), and <em>N</em>-folate-3-aminopropyl (FAP). In contrast to the ζ-potential of −18.4 mV for FITC-MSN, the values of ζ-potential for AP-, GP-, GEGP-, and FAP-functionalized FITC-MSNs in 100 mM PBS buffer (pH 7.4) increased positively from −11.3, −10.6, −4.0, to +4.9 mV, respectively. The uptake efficiency, endocytosis mechanism, and biocompatibility of these organically functionalized MSNs were investigated with human cervical cancer cells (HeLa). Flow cytometry results suggested that the endocytosis of MSN could be manipulated by different surface functionalization. The immunocytochemistry study indicated that the uptake of these MSNs by HeLa cells was surface functional group dependent and involved several different mechanisms of endocytosis. Confocal fluorescence micrographs showed that the different surface functionalities of MSNs could also affect their ability to escape endosomal entrapment, which is a key factor in designing effective intracellular delivery vehicles.</p>
dc.description.comments <p>Reprinted (adapted) with permission from <em>Journal of the American Chemical Society </em> 128 (2006): 14792, doi:<a href="http://dx.doi.org/10.1021/ja0645943" target="_blank">10.1021/ja0645943</a>. Copyright 2006 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/chem_pubs/130/
dc.identifier.articleid 1131
dc.identifier.contextkey 7881690
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath chem_pubs/130
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/14563
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/chem_pubs/130/0-2007_Slowing_LicenseEffectSurface.pdf|||Fri Jan 14 19:41:49 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/chem_pubs/130/2007_Slowing_EffectSurface.pdf|||Fri Jan 14 19:41:51 UTC 2022
dc.source.uri 10.1021/ja0645943
dc.subject.disciplines Chemistry
dc.title Effect of Surface Functionalization of MCM-41-Type Mesoporous Silica Nanoparticles on the Endocytosis by Human Cancer Cells
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 15e8ccb1-3931-4bf0-bd09-3586ad3c87a9
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
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