Sorghum is one of the most widely grown cereal crops in the world. It is used for grain production for human and livestock consumption and biomass production for use as a biofuel source. Lignin is one of the main carbon-based structural components in plant cell walls. Lignin plays a role in plant stalk strength and digestibility of plant tissue. In the study detailed here, multiple replicates of 300 different lines of sorghum were grown in two fields, and the lignin concentration of these lines was compared in order to test for environmental effects. Lignin production of photoperiod-sensitive genotypes against photoperiod-insensitive genotypes was also compared. It was found that replicates of the same genotype grown in different fields produced significantly different concentration of lignin and that photoperiod-sensitive genotypes had a significantly lower concentration of lignin compared to photoperiod-insensitive genotypes.