Development of an RNA aptamer to PD173955N through SELEX

dc.contributor.advisor Marit Nilsen-Hamilton
dc.contributor.advisor Amy Andreotti
dc.contributor.advisor W. A. Miller
dc.contributor.author Mehanovic, Samir
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2018-08-23T07:26:01.000
dc.date.accessioned 2020-06-30T07:40:34Z
dc.date.available 2020-06-30T07:40:34Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2008
dc.date.issued 2008-01-01
dc.description.abstract <p>With all the technology available to us, the causes of cancers are still puzzling researchers all over the world. Everyday different approaches are being tested to help cure various cancers. With the development of molecular biology, we are able to understand cancer more at molecular level, which helps us look at different methods to treat cancer patients.;The molecular basis for leukemia cancers are understood to a level where we are able to design treatments that specifically target proteins responsible for the disease. One of those proteins is the Bcr-Abl tyrosine kinase which is mostly responsible for chronic myelogenous leukemia. PD173955 is a drug that is capable of inhibiting Bcr-Abl tyrosine kinase more potently than any other chemotherapeutic drug reported drug. The problem with PD173955 and a very large number of other drugs is their limited solubility in aqueous environments which makes them less desirable for medical applications.;Aptamers are newly developed reagents and their utilization in medicinal applications is so far very limited. However their characteristics make them perfect tools to help solve problems associated with PD173955. Here we report a successful selection of the RNA aptamer that is capable of binding PD173955 with a dissociation constant of 1-2 muM using SELEX. The data obtained with selected aptamer suggests that the aptamer could be used to protect PD173955 from aqueous environment. The selection process of the RNA aptamer will be described in detail. The selected aptamer has been reduced to its minimal binding domain without loss of affinity.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/14936/
dc.identifier.articleid 15935
dc.identifier.contextkey 7008128
dc.identifier.doi https://doi.org/10.31274/rtd-180813-16079
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/14936
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/68516
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/14936/1453070.PDF|||Fri Jan 14 20:28:59 UTC 2022
dc.subject.disciplines Biochemistry
dc.subject.keywords Biochemistry
dc.subject.keywords biophysics
dc.subject.keywords and molecular biology;Biochemistry;
dc.title Development of an RNA aptamer to PD173955N through SELEX
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication faf0a6cb-16ca-421c-8f48-9fbbd7bc3747
thesis.degree.level thesis
thesis.degree.name Master of Science
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