Transcriptional regulation of mosquito oogenesis

dc.contributor.advisor Lyric C. Bartholomay
dc.contributor.author Jennings, Patrick
dc.contributor.department Entomology
dc.date 2018-08-11T09:56:08.000
dc.date.accessioned 2020-06-30T02:40:53Z
dc.date.available 2020-06-30T02:40:53Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2011
dc.date.embargo 2013-06-05
dc.date.issued 2011-01-01
dc.description.abstract <p>The Eastern treehole mosquito, <i>Aedes triseriatus</i> (Say), is the primary vector of La Crosse virus (LACV) in the United States. LACV is maintained in natural populations in part by transovarial transmission from infected females to their progeny. Our understanding of the innate immune response in mosquito ovaries is a fundamental step toward understanding the transmission cycle of LACV and other vertically transmitted arthropod-borne viruses. We investigated signaling mechanisms between the nutrient content of the vertebrate blood meal and activation of mosquito egg development. In particular, the target of rapamycin (TOR) serine/threonine protein kinase is a central regulator of nutritional signaling, and we examined the roles of TOR and its downstream target, S6K, in <i>Ae. triseriatus</i> by RNA interference. We also examined the transcriptomes of <i>Ae. triseriatus</i> and <i>Ae. aegypti</i> (L.) ovaries by Illumina RNA-seq. We assembled 17,743 novel transcript sequences from <i>Ae. triseriatus</i> ovary mRNA using a novel and highly accurate assembly method, and we identified genes that are differentially regulated during the processes of follicular atresia, apoptosis, and autophagy. Distinct differences exist between the transcriptional regulation of <i>Ae. triseriatus</i> and <i>Ae. aegypti</i> oogenesis. TOR and many other proteins with previously unknown function are transcriptionally regulated during apoptosis and autophagy in mosquito ovaries during oogenesis.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/12181/
dc.identifier.articleid 3199
dc.identifier.contextkey 2808397
dc.identifier.doi https://doi.org/10.31274/etd-180810-1475
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/12181
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/26374
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/12181/Jennings_iastate_0097M_11970.pdf|||Fri Jan 14 19:14:43 UTC 2022
dc.subject.disciplines Entomology
dc.subject.keywords mosquito
dc.subject.keywords nutrition signaling
dc.subject.keywords RNA-seq
dc.subject.keywords transcriptome
dc.title Transcriptional regulation of mosquito oogenesis
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
thesis.degree.level thesis
thesis.degree.name Master of Science
File
Original bundle
Now showing 1 - 1 of 1
Name:
Jennings_iastate_0097M_11970.pdf
Size:
1.9 MB
Format:
Adobe Portable Document Format
Description: