Assembly of the 30S ribosomal subunit: positioning of ribosomal protein S13 in the S7 assembly branch

dc.contributor.author Grondek, Joel
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2020-11-06T02:25:14.000
dc.date.accessioned 2021-02-26T08:53:02Z
dc.date.available 2021-02-26T08:53:02Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2004
dc.date.issued 2004-01-01
dc.description.abstract <p>Studies of Escherichia coli 30S ribosomal subunit assembly have revealed a hierarchical and cooperative association of ribosomal proteins with 16S ribosomal RNA; these results have been used to compile an in vitro 30S subunit assembly map. In single protein addition and omission studies, ribosomal protein S13 was shown to be dependent on the prior association of ribosomal protein S20 for binding to the ribonucleoprotein particle. While the overwhelming majority of interactions revealed in the assembly map are consistent with additional data, the dependency of S13 on S20 is not. Structural studies position S13 in the head of the 30S subunit over 100Å away from S20, which resides near the bottom of the body of the 30S subunit. All of the proteins that reside in the head of the 30S subunit, except S13, have been shown to be part of the S7 assembly branch, that is, they all depend on S7 for association with the assembling 30S subunit. Given these observations, the assembly requirements for S13 were investigated using base-specific chemical footprinting and primer extension analysis. These studies reveal that S13 can bind to 16S rRNA in the presence of S7, but not S20. Additionally, polyspecific interaction between S13 and other members of the S7 assembly branch have been observed. These results link S13 to the 3' major domain family of proteins, and the S7 assembly branch, placing S13 in a new location in the 30S subunit assembly map where its position is in accordance with much biochemical and structural data.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/20439/
dc.identifier.articleid 21438
dc.identifier.contextkey 19953175
dc.identifier.doi https://doi.org/10.31274/rtd-20201023-113
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/20439
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/97806
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/20439/Grondek_ISU_2004_G758.pdf|||Fri Jan 14 22:24:59 UTC 2022
dc.subject.keywords Biochemistry
dc.subject.keywords Biochemistry, biophysics, and molecular biology
dc.title Assembly of the 30S ribosomal subunit: positioning of ribosomal protein S13 in the S7 assembly branch
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication faf0a6cb-16ca-421c-8f48-9fbbd7bc3747
thesis.degree.discipline Biochemistry
thesis.degree.level thesis
thesis.degree.name Master of Science
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