Evaluating suspected CWD feral pig brain samples using RT-QuIC and protocol for the purification and quality control of recombinant monomeric proteins for RT-QuIC assay

dc.contributor.advisor Anumantha Kanthasamy
dc.contributor.author Hepker, Monica
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2019-09-20T04:05:27.000
dc.date.accessioned 2020-06-30T03:16:40Z
dc.date.available 2020-06-30T03:16:40Z
dc.date.copyright Wed May 01 00:00:00 UTC 2019
dc.date.embargo 2021-04-23
dc.date.issued 2019-01-01
dc.description.abstract <p>Proteinopathy is a broad term used to describe any disease or condition that arises as</p> <p>a result of aberrant protein behavior. Most often it is used when discussing any of a wide</p> <p>range of neurodegenerative diseases that tend to result from an abnormal misfolding and</p> <p>subsequent aggregation of a normal cellular protein. The pathology of each of these diseases</p> <p>is largely dependent upon the nature of the protein aggregate that is responsible for or</p> <p>resulting from the disease. When viewed individually each disease seems only vaguely</p> <p>similar to others, but when a step back is taken to consider the broader aspects of the disease</p> <p>it begins to show similarities with other diseases, some of which are better studied and</p> <p>understood. The advances in other proteinopathies can then be experimentally applied to a</p> <p>specific disease of interest and the results compared to gain better understand the</p> <p>mechanisms underlying its pathogenesis and progression.</p> <p>Two examples of such proteinopathies are transmissible spongiform encephalopathies</p> <p>(TSE) and Parkinson’s disease (PD). TSEs are a subset of prion misfolding diseases,</p> <p>specifically identified as being transmissible to other individuals in a manner resembling</p> <p>viral, bacterial, or toxic exposure, that have been studied in animals for decades. While rare</p> <p>in humans, prion protein misfolding does occur in a handful of different forms. PD on the</p> <p>other hand is fairly common in older individuals, affecting approximately one million people</p> <p>in the United States and an estimated 10 million people worldwide. Both conditions share</p> <p>such notable traits as characteristic protein aggregates, being neurodegenerative, eventually</p> <p>fatally so, and having a link to the enteric nervous system. Techniques applied to the study of</p> <p>TSEs have been successfully applied to the study of PD, and inverse has occurred as well,</p> <p>leading to leap frog effect of advancement for both diseases.</p> <p>In this thesis, topics of two proteinopathies are covered. A literature review of chronic</p> <p>wasting disease (CWD) history, study, and further interests leads the paper. This is followed</p> <p>by a chapter on analysis of feral pig brainstem by RT-QuIC to determine the presence or</p> <p>absence of aggregated prion protein. The next chapter is dedicated to describing a protocol</p> <p>for the efficient and reliable production of recombinant human α-synuclein monomeric</p> <p>protein for use in research. Each of these techniques has been applied to the study of multiple</p> <p>diseases, but the refinement of each technique for application to the disease or species of</p> <p>interest is essential to achieving the best results.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/17202/
dc.identifier.articleid 8209
dc.identifier.contextkey 15016118
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/17202
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/31385
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/17202/Hepker_iastate_0097M_18078.pdf|||Fri Jan 14 21:18:39 UTC 2022
dc.subject.disciplines Pharmacology
dc.subject.keywords CWD
dc.subject.keywords Neurodegenerative
dc.subject.keywords Protein
dc.subject.keywords Proteinopathy
dc.subject.keywords RT-QuIC
dc.subject.keywords TSE
dc.title Evaluating suspected CWD feral pig brain samples using RT-QuIC and protocol for the purification and quality control of recombinant monomeric proteins for RT-QuIC assay
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
thesis.degree.discipline Biomedical Sciences
thesis.degree.level thesis
thesis.degree.name Master of Science
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