Inheritance and fitness costs Of field-derived resistance To Cry3Bb1 corn by western corn rootworm

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dc.contributor.advisor Aaron J. Gassmann
dc.contributor.author Paolino, Aubrey
dc.contributor.department Entomology
dc.date 2018-08-11T20:00:50.000
dc.date.accessioned 2020-06-30T03:01:19Z
dc.date.available 2020-06-30T03:01:19Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.embargo 2001-01-01
dc.date.issued 2016-01-01
dc.description.abstract <p>The western corn rootworm (Diabrotica virgifera virgifera LeConte) is serious pest of corn, particularly in the Midwestern United States. One strategy used to manage western corn rootworm is the planting of transgenic corn that produces one or more Cry toxins derived from Bacillus thuringiensis (Bt). Refuges of non-Bt corn function to delay the development of resistance to these toxins. The greatest delay in resistance development is expected when the inheritance of resistance is recessive and there are associated fitness costs. We characterized the inheritance of resistance of two strains of western corn rootworm with field-derived resistance to the Bt toxin Cry3Bb1 (Elma and Monona) and tested for fitness costs of resistance. The inheritance of resistance to Cry3Bb1 was investigated by crossing resistant and susceptible western corn rootworm and evaluating the survival of their progeny on Bt using diet-based assays and experiments with whole plants and seedling mats. For both strains, resistance was found to be non-recessive. In a greenhouse experiment in which larvae were reared on whole corn plants in field soil, no fitness costs of resistance were detected for Monona. In a laboratory experiment with Elma, in which larvae experienced intraspecific and interspecific competition for food, a fitness cost of delayed larval development was identified, however, no other fitness costs were found. These findings underscore the need for rootworm resistance management beyond the refuge strategy. Multiple tactics such as crop rotation, the planting of non-Bt corn with soil-applied insecticide, and planting pyramids of multiple Bt toxins that target western corn rootworm must be used to maintain the effectiveness of available Bt toxins.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/15085/
dc.identifier.articleid 6092
dc.identifier.contextkey 8882642
dc.identifier.doi https://doi.org/10.31274/etd-180810-4687
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/15085
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/29269
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/15085/Paolino_iastate_0097M_15618.pdf|||Fri Jan 14 20:35:27 UTC 2022
dc.subject.disciplines Entomology
dc.subject.keywords Entomology
dc.subject.keywords Bacillus thuringiensis
dc.subject.keywords Bt corn
dc.subject.keywords Diabrotica virgifera virgifera
dc.subject.keywords refuge strategy
dc.subject.keywords resistance management
dc.title Inheritance and fitness costs Of field-derived resistance To Cry3Bb1 corn by western corn rootworm
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
thesis.degree.discipline Entomology
thesis.degree.level thesis
thesis.degree.name Master of Science
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