Site-directed mutagenesis and PBAN activation of the Helicoverpa zea PBAN-receptor

dc.contributor.author Choi, Man-Yeon
dc.contributor.author Jurenka, Russell
dc.contributor.author Jurenka, Russell
dc.contributor.department Entomology
dc.date 2018-02-15T03:05:50.000
dc.date.accessioned 2020-06-30T02:23:01Z
dc.date.available 2020-06-30T02:23:01Z
dc.date.embargo 2010-01-01
dc.date.issued 2010-03-01
dc.description.abstract <p>Pheromone biosynthesis-activating neuropeptide (PBAN) and pyrokinins belong to a family of insect peptide hormones that have a common FXPRLamide C-terminal ending. The G-protein-coupled receptors (GPCRs) for this peptide family were first identified from a moth and <em>Drosophila</em> with sequence similarity to neuromedin U receptors from vertebrates. We have characterized the PBAN-receptor (PBAN-R or PR) active binding domains using chimeric GPCRs and proposed that extracellular loop 3 is critical for ligand selection. Here, we characterized the 3rd extracellular domain of PBAN-R through site-directed point mutations. Results are discussed in context of the structural features required for receptor activation using receptor activation experiments and in silico computational modeling. This research will help in characterizing these receptors towards a goal of finding agonists and/or antagonists for PBAN/pyrokinin receptors.</p>
dc.description.comments <p>This article is from <em>FEBS Letters </em>584 (2010): 1212, doi:<a href="Http://dx.doi.org/10.1016/j.febslet.2010.02.033" target="_blank">10.1016/j.febslet.2010.02.033</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ent_pubs/275/
dc.identifier.articleid 1274
dc.identifier.contextkey 6244391
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/275
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/23889
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/275/2010_Jurenka_SiteDirected.pdf|||Fri Jan 14 23:07:21 UTC 2022
dc.source.uri 10.1016/j.febslet.2010.02.033
dc.subject.disciplines Biochemistry
dc.subject.disciplines Entomology
dc.subject.disciplines Genetics
dc.subject.keywords PBAN-receptor
dc.subject.keywords GPCR
dc.subject.keywords Point mutation
dc.subject.keywords Extracellular domain
dc.subject.keywords Pyrokinin
dc.title Site-directed mutagenesis and PBAN activation of the Helicoverpa zea PBAN-receptor
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication e08b5f3a-0eb8-4424-92e6-75158e5a7c4d
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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