Genetic architecture of gene expression underlying variation in host response to porcine reproductive and respiratory syndrome virus infection

dc.contributor.author Kommadath, Arun
dc.contributor.author Reecy, James
dc.contributor.author Bao, Hua
dc.contributor.author Choi, Igseo
dc.contributor.author Reecy, James
dc.contributor.author Koltes, James
dc.contributor.author Fritz-Waters, Elyn
dc.contributor.author Eisley, Chris
dc.contributor.author Grant, Jason
dc.contributor.author Rowland, Robert
dc.contributor.author Tuggle, Christopher
dc.contributor.author Dekkers, Jack
dc.contributor.author Lunney, Joan
dc.contributor.author Guan, Le Luo
dc.contributor.author Stothard, Paul
dc.contributor.author Plastow, Graham
dc.contributor.department Animal Science
dc.contributor.department Statistics
dc.date 2019-04-22T07:52:12.000
dc.date.accessioned 2020-06-29T23:40:44Z
dc.date.available 2020-06-29T23:40:44Z
dc.date.issued 2017-01-01
dc.description.abstract <p>It has been shown that inter-individual variation in host response to porcine reproductive and respiratory syndrome (PRRS) has a heritable component, yet little is known about the underlying genetic architecture of gene expression in response to PRRS virus (PRRSV) infection. Here, we integrated genome-wide genotype, gene expression, viremia level, and weight gain data to identify genetic polymorphisms that are associated with variation in inter-individual gene expression and response to PRRSV infection in pigs. RNA-seq analysis of peripheral blood samples collected just prior to experimental challenge (day 0) and at 4, 7, 11 and 14 days post infection from 44 pigs revealed 6,430 differentially expressed genes at one or more time points post infection compared to the day 0 baseline. We mapped genetic polymorphisms that were associated with inter-individual differences in expression at each day and found evidence of <em>cis-</em>acting expression quantitative trait loci (<em>cis-</em>eQTL) for 869 expressed genes (qval < 0.05). Associations between <em>cis</em>-eQTL markers and host response phenotypes using 383 pigs suggest that host genotype-dependent differences in expression of <em>GBP5, GBP6, CCHCR1 and CMPK2</em> affect viremia levels or weight gain in response to PRRSV infection.</p>
dc.description.comments <p>This article is published as Kommadath, Arun, Hua Bao, Igseo Choi, James M. Reecy, James E. Koltes, Elyn Fritz-Waters, Chris J. Eisley et al. "Genetic architecture of gene expression underlying variation in host response to porcine reproductive and respiratory syndrome virus infection." <em>Scientific reports</em> 7 (2017): 46203. doi: <a href="https://doi.org/10.1038/srep46203">10.1038/srep46203</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/425/
dc.identifier.articleid 1429
dc.identifier.contextkey 14061173
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/425
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9852
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/425/2017_Reecy_GeneticArchitecture.pdf|||Sat Jan 15 00:13:47 UTC 2022
dc.source.uri 10.1038/srep46203
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.title Genetic architecture of gene expression underlying variation in host response to porcine reproductive and respiratory syndrome virus infection
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
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