Somatostatin inhibits insulin release via SSTR2 in the hamster pancreatic islets

dc.contributor.author Yao, Cheng-Yu
dc.contributor.department Animal Science
dc.date 2018-08-22T17:07:53.000
dc.date.accessioned 2020-06-30T08:00:37Z
dc.date.available 2020-06-30T08:00:37Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2004
dc.date.issued 2004-01-01
dc.description.abstract <p>It is generally accepted that somatostatin receptor subtype 5 (SSTR5) mediates the inhibition of insulin release. This is due to the fact that in the human, rat, and mouse somatostatin (SST) inhibits insulin release via SSTR5; however, in a preliminary study from Dr. Hsu's laboratory with HIT-T15 [beta]-cells that are derived from hamster insulinoma, it was found that a SSTR2 agonist, but not other SSTR agonists, inhibited insulin release. Since SSTR2 is highly expressed in tumor cells, it is possible that the results from HIT-T15 cells may be due to the nature of tumor cells. Thus, it is hypothesized that in hamsters, SSTR2 mediates somatostatin-induced inhibition of insulin release. Isolated hamster pancreatic islets were used in the present study to test this hypothesis. Both somatostatin (1-100 nM) and nonpeptide SSTR2 agonist L-779,976 (0.1-100 nM) inhibited insulin release in a dose-dependent manner. Nonpeptide agonists for SSTR1, 3, 4, and 5 at the highest concentration studied (1[mu]M) failed to inhibit insulin release. PRL-2903, a peptide SSTR2 antagonist (0.3-1[mu]M), antagonized somatostatin-induced inhibition of insulin release in a dose-dependent manner. It is concluded that in hamster [beta]-cells, somatostatin inhibits insulin release via SSTR2 but not SSTR5.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/17520/
dc.identifier.articleid 18520
dc.identifier.contextkey 12371000
dc.identifier.doi https://doi.org/10.31274/rtd-180813-8302
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/17520
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/71343
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/17520/Yao_ISU_2004_Y37.pdf|||Fri Jan 14 21:24:54 UTC 2022
dc.subject.keywords Animal Physiology
dc.title Somatostatin inhibits insulin release via SSTR2 in the hamster pancreatic islets
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
thesis.degree.discipline Animal Physiology
thesis.degree.level thesis
thesis.degree.name Master of Science
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