Identification of surface accessible proteins in parasitic nematode extracellular vesicles

Thumbnail Image
File
Sondjaja_iastate_0097M_21411.pdf (5.74 MB)

File Embargoed Until: (2026-06-07)

Sondjaja_iastate_0097M_23/Supplemental information for Manuscript.docx (47.83 KB)

File Embargoed Until: (2026-06-07)
Supplemental Files
Sondjaja_iastate_0097M_21411.pdf (5.74 MB)

File Embargoed Until: (2026-06-07)

Sondjaja_iastate_0097M_23/Supplemental information for Manuscript.docx (47.83 KB)

File Embargoed Until: (2026-06-07)
Date
2024-05
Authors
Sondjaja, Noelle Angelique
Major Professor
Advisor
Kimber, Michael J
Day, Timothy
Verhoeven, David
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Journal Issue
Is Version Of
Versions
Series
Department
Veterinary Biomedical Sciences
Abstract
Extracellular vesicles (EVs) are membrane-bound nanoparticles released by the cells of many organisms, containing important biological molecules including bioactive proteins, lipids, and nucleic acids. EVs are shed by parasitic nematodes into the host environment and have been shown to play a crucial role in establishment and maintenance of chronic infection through their interaction with host cells, these EVs transmit information and play an important immunomodulatory role at the parasite-host interface. Along with their role in the establishment and maintenance of chronic infection, parasitic EVs are potential biomarkers of infection due to the species, sex, and life stage specificity of their cargo and structural composition. The exploitation of parasite EVs as a novel diagnostic tool is of particular importance in controlling parasitic nematode diseases. Helminth infections, including dirofilariasis and ascariasis, are a global health concern, but current diagnostic tools for these infections are invasive and suboptimal. Leveraging parasitic nematode EVs to address this challenge is appealing, but progress in identifying effective protein biomarkers in parasitic nematode EVs has been hindered by our general lack of descriptive information on their proteomes and spatial information on which their proteins are accessible to current diagnostic approaches. Here, we adopt a multiple-proteomic approach to analyze Ascaris suum and Dirofilaria immitis EV proteomes and identify proteins containing transmembrane helices, as surface accessible peptides present a clear target with biomarker potential. Our data is necessary in understanding biological function of EVs in recipient cells and unlocking the utilization of EVs as diagnostic biomarkers.
Comments
Description
Keywords
Citation
Source
Subject Categories
Copyright