Structural differences and differential expression among rhabdomeric opsins reveal functional change after gene duplication in the bay scallop, Argopecten irradians (Pectinidae)

dc.contributor.author Porath-Krause, Anita
dc.contributor.author Pairett, Autum
dc.contributor.author Serb, Jeanne
dc.contributor.author Faggionato, Davide
dc.contributor.author Birla, Bhagyashree
dc.contributor.author Sankar, Kannan
dc.contributor.author Serb, Jeanne
dc.contributor.department Ecology, Evolution and Organismal Biology
dc.date 2018-02-19T06:01:08.000
dc.date.accessioned 2020-06-30T02:17:26Z
dc.date.available 2020-06-30T02:17:26Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-01-01
dc.description.abstract <p><h3>Background</h3> <p id="x-x-x-Par1">Opsins are the only class of proteins used for light perception in image-forming eyes. Gene duplication and subsequent functional divergence of opsins have played an important role in expanding photoreceptive capabilities of organisms by altering what wavelengths of light are absorbed by photoreceptors (spectral tuning). However, new opsin copies may also acquire novel function or subdivide ancestral functions through changes to temporal, spatial or the level of gene expression. Here, we test how opsin gene copies diversify in function and evolutionary fate by characterizing four rhabdomeric (G<sub>q</sub>-protein coupled) opsins in the scallop, <em>Argopecten irradians</em>, identified from tissue-specific transcriptomes. <h3>Results</h3> <p id="x-x-x-Par2">Under a phylogenetic analysis, we recovered a pattern consistent with two rounds of duplication that generated the genetic diversity of scallop G<sub>q</sub>-opsins. We found strong support for differential expression of paralogous G<sub>q</sub>-opsins across ocular and extra-ocular photosensitive tissues, suggesting that scallop G<sub>q</sub>-opsins are used in different biological contexts due to molecular alternations outside and within the protein-coding regions. Finally, we used available protein models to predict which amino acid residues interact with the light-absorbing chromophore. Variation in these residues suggests that the four G<sub>q</sub>-opsin paralogs absorb different wavelengths of light. <h3>Conclusions</h3> <p id="x-x-x-Par3">Our results uncover novel genetic and functional diversity in the light-sensing structures of the scallop, demonstrating the complicated nature of G<sub>q</sub>-opsin diversification after gene duplication. Our results highlight a change in the nearly ubiquitous shadow response in molluscs to a narrowed functional specificity for visual processes in the eyed scallop. Our findings provide a starting point to study how gene duplication may coincide with eye evolution, and more specifically, different ways neofunctionalization of G<sub>q</sub>-opsins may occur.</p>
dc.description.comments <p>This article is published as Porath-Krause, Anita J., Autum N. Pairett, Davide Faggionato, Bhagyashree S. Birla, Kannan Sankar, and Jeanne M. Serb. "Structural differences and differential expression among rhabdomeric opsins reveal functional change after gene duplication in the bay scallop, Argopecten irradians (Pectinidae)." BMC evolutionary biology 16, no. 1 (2016): 250. doi: <a href="https://doi.org/10.1186/s12862-016-0823-9" target="_blank">10.1186/s12862-016-0823-9</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/eeob_ag_pubs/239/
dc.identifier.articleid 1243
dc.identifier.contextkey 11219432
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath eeob_ag_pubs/239
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/23111
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/eeob_ag_pubs/239/2016_Serb_StructuralDifferences.pdf|||Fri Jan 14 22:50:02 UTC 2022
dc.source.uri 10.1186/s12862-016-0823-9
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Behavior and Ethology
dc.subject.disciplines Ecology and Evolutionary Biology
dc.subject.disciplines Evolution
dc.subject.disciplines Terrestrial and Aquatic Ecology
dc.subject.keywords Rhabdomeric photoreceptor
dc.subject.keywords R-opsin
dc.subject.keywords Gene duplication
dc.subject.keywords Melanopsin
dc.subject.keywords Rhodopsin
dc.subject.keywords Vision
dc.title Structural differences and differential expression among rhabdomeric opsins reveal functional change after gene duplication in the bay scallop, Argopecten irradians (Pectinidae)
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 0d7c4c16-b4be-426c-9439-e7985e654bce
relation.isOrgUnitOfPublication 6fa4d3a0-d4c9-4940-945f-9e5923aed691
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