Lymphocyte populations and their relationship to cell-mediated immune responses in aflatoxin-treated guinea pigs

dc.contributor.author McLoughlin, Mary
dc.contributor.department Theses & dissertations (Interdisciplinary)
dc.date 2018-08-15T22:45:57.000
dc.date.accessioned 2020-07-02T06:06:26Z
dc.date.available 2020-07-02T06:06:26Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 1982
dc.date.issued 1982
dc.description.abstract <p>Effects of aflatoxin on cell-mediated immunity were investigated in guinea pigs. Dose dependent reductions in skin test responses were observed in guinea pigs which received at least 0.040 mg/kg/day aflatoxin B(,1) equivalents (B(,1) eq) for 3 weeks. Reduced weight gains were apparent at the 0.020 mg/kg/day B(,1) eq level. Serum bile acids were elevated at low levels of aflatoxin ingestion, in guinea pigs which displayed normal skin test responsiveness and weight gain;Passive transfer of delayed type hypersensitivity (DTH) was used to evaluate T lymphocytes or macrophages from aflatoxin-treated guinea pigs. The skin test responses of both nontreated guinea pigs receiving peritoneal exudate cells (PEC) from aflatoxin-treated guinea pigs and aflatoxin-treated guinea pigs receiving PEC from nontreated guinea pigs were numerically lower, but not significantly different (P < 0.05), than that of controls. Variability in susceptibility to aflatoxin was noted among individuals within groups;A bacterial binding assay currently used to identify human lymphocytes was adapted for use in guinea pigs. Three lymphocyte markers were identified: Salmonella schottmulleri and Yersinia enterocolitica, which appear to label all or some guinea pig T lymphocytes in peripheral blood; and Brucella melitensis, identified as a marker for guinea pig B lymphocytes in peripheral blood. Lymphocytes from spleen, thymus, and lymph node had different binding capacities than lymphocytes in peripheral blood, reflecting perhaps maturational differences among lymphocytes from different sources;Lymphocytes from nontreated and aflatoxin-treated guinea pigs were counted using immunofluorescence and bacterial markers. No changes occurred in absolute number of B or T lymphocytes identified by immunofluorescence. The number of S schottmulleri-positive lymphocytes closely approximated the numbr of T lymphocytes identified by immunofluorescence, except at the highest dose of aflatoxin used. At this level (0.060 mg/kg/day B(,1) eq) some T lymphocytes identified by immunofluorescence were refractory to binding of S schottmulleri;Aflatoxin impaired skin test responsiveness and passive transfer of DTH without causing quantitative changes in T or B lymphocyte populations in peripheral blood.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/8369/
dc.identifier.articleid 9368
dc.identifier.contextkey 6333725
dc.identifier.doi https://doi.org/10.31274/rtd-180813-1801
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/8369
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/81349
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/8369/r_8307772.pdf|||Sat Jan 15 02:10:21 UTC 2022
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Veterinary Medicine
dc.subject.keywords Immunobiology
dc.title Lymphocyte populations and their relationship to cell-mediated immune responses in aflatoxin-treated guinea pigs
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
thesis.degree.discipline Immunobiology
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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