Toxicity of chlorpyrifos adsorbed on clay and humic acid to larval walleye

dc.contributor.advisor Robert C. Summerfelt
dc.contributor.advisor Gary J. Atchison
dc.contributor.author Phillips, Todd
dc.contributor.department Animal Ecology
dc.date 2018-08-23T12:09:45.000
dc.date.accessioned 2020-06-30T07:32:35Z
dc.date.available 2020-06-30T07:32:35Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2000
dc.date.issued 2000-01-01
dc.description.abstract <p>Agricultural production poses one of the most serious threats to the continued ecological integrity of environmental systems and has been charged as the activity most responsible for loss of fish species in streams. Although all anthropogenic stressors affecting aquatic ecosystems have not been isolated, suspended solids, sediment, and pesticides are believed to be major factors. The objectives of this dissertation were to: (1) evaluate sources of variability in measuring cholinesterase activity, (2) determine the most sensitive life stage of walleye exposed to chlorpyrifos, (3) evaluate the bioavailability of chlorpyrifos adsorbed on humic acid (HA) and Panther Creek (PC) clay to larval walleye (Stizostedion vitreum), and (4) determine the toxicity of Cedar River sediments and water to larval walleye. Water temperature, stress, and the method of euthanasia had no effect on ChE activity of walleye. ChE activity of walleye stored at -80°C for 180 d did not differ from ChE activity of fresh specimens. However, a significant positive relationship was observed between whole body ChE activity and total length of larval walleye, but no significant relationship was found between total length of juvenile walleye and brain ChE activity. These results indicate that ChE inhibition is a reliable and sensitive indicator of OP exposure to walleye and is not affected by environmental factors. Prolarvae (yolk-sac larvae) were the least sensitive larval stage, but there was a significant increase in toxicity of chlorpyrifos from the postlarvae I to postlarvae II stage. The LC50 leveled out in 30 to 90-d-old juvenile walleye. Chlorpyrifos adsorbed on humic acid reduced survival of larval walleye indicating that chlorpyrifos desorbs from HA. Thus, chlorpyrifos-HA complexes represent a potential exposure route for OPs to fish. Also, PC clay alone, without chlorpyrifos, was highly toxic to postlarvae I and postlarvae II walleye, but not prolarvae. Neither Cedar River water nor sediment were more toxic to larval walleye, at any larval stage, than reference water and sediment. These results suggest that Cedar River sediment and water do not pose a serious threat to larval walleye in the Cedar River.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/13921/
dc.identifier.articleid 14920
dc.identifier.contextkey 6950768
dc.identifier.doi https://doi.org/10.31274/rtd-180813-15275
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/13921
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/67448
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/13921/r_9962837.pdf|||Fri Jan 14 20:04:14 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Aquaculture and Fisheries
dc.subject.disciplines Environmental Sciences
dc.subject.disciplines Fresh Water Studies
dc.subject.keywords Animal ecology
dc.subject.keywords Toxicology
dc.subject.keywords Fisheries biology
dc.title Toxicity of chlorpyrifos adsorbed on clay and humic acid to larval walleye
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication dc916ec7-70d9-48fc-a9b4-83f345e17b12
thesis.degree.discipline Toxicology
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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