Single dose combination nanovaccine provides protection against influenza A virus in young and aged mice

Date
2019-01-01
Authors
Narasimhan, Balaji
Ross, Kathleen
Senapati, Sujata
Alley, Jessica
Darling, Ross
Goodman, Jonathan
Jefferson, Matthew
Uz, Metin
Mallapragada, Surya
Guo, Baoqing
Yoon, Kyoung-Jin
Verhoeven, David
Kohut, Marian
Mallapragada, Surya
Wannemuehler, Michael
Narasimhan, Balaji
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Ames Laboratory
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Neuroscience
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Kinesiology
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Ames LaboratoryNeuroscienceKinesiologyVeterinary Microbiology and Preventive MedicineMaterials Science and EngineeringChemical and Biological EngineeringVeterinary Diagnostic and Production Animal MedicineAmes Laboratory
Abstract

Immunosenescence poses a formidable challenge in designing effective influenza vaccines for aging populations. While approved vaccines against influenza viruses exist, their efficacy in older adults is significantly decreased due to the diminished capabilities of innate and adaptive immune responses. In this work, the ability of a combination nanovaccine containing both recombinant hemagglutinin and nucleoprotein to provide protection against seasonal influenza virus infection was examined in young and aged mice. Vaccine formulations combining two nanoadjuvants, polyanhydride nanoparticles and pentablock copolymer micelles, were shown to enhance protection against challenge compared to each adjuvant alone in young mice. Nanoparticles were shown to enhance in vitro activation of dendritic cells isolated from aged mice, while both nanoadjuvants did not induce proinflammatory cytokine secretion which may be detrimental in aged individuals. In addition, the combination nanovaccine platform was shown to induce demonstrable antibody titers in both young and aged mice that correlated with the maintenance of body weight post-challenge. Collectively, these data demonstrate that the combination nanovaccine platform is a promising technology for influenza vaccines for older adults.

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This is a manuscript of an article published as Ross, Kathleen A., Sujata Senapati, Jessica R. Alley, Ross J. Darling, Jonathan Goodman, Matthew Jefferson, Metin Uz, Baoqing Guo , Kyoung-Jin Yoon , David E Verhoeven , Marian L. Kohut , Surya K. Mallapragada, Michael J. Wannemuehler and Balaji Narasimhan. "Single dose combination nanovaccine provides protection against influenza A virus in young and aged mice." Biomaterials Science (2019). doi: 10.1039/C8BM01443D. Posted with permission.

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