Seasonal variations in export of antibiotic resistance genes and bacteria in runoff from an agricultural watershed in Iowa

dc.contributor.author Soupir, Michelle
dc.contributor.author Neher, Timothy
dc.contributor.author Ma, Lanying
dc.contributor.author Moorman, Thomas
dc.contributor.author Howe, Adina
dc.contributor.author Soupir, Michelle
dc.contributor.author Howe, Adina
dc.contributor.department Civil, Construction and Environmental Engineering
dc.contributor.department Bioinformatics and Computational Biology
dc.contributor.department Agricultural and Biosystems Engineering
dc.date 2020-06-23T17:25:48.000
dc.date.accessioned 2020-06-29T22:37:13Z
dc.date.available 2020-06-29T22:37:13Z
dc.date.issued 2020-06-16
dc.description.abstract <p>Seasonal variations of antimicrobial resistance (AMR) indicators in runoff water can help improve our understanding of AMR sources and transport within an agricultural watershed. This study aimed to monitor multiple areas throughout the Black Hawk Lake (BHL) watershed (5324 ha) in central Iowa during 2017 and 2018 that consists of both swine and cattle feeding operations as well as known areas with manure application. The measured indicators included plate counts for fecal indicator bacteria (FIB) <em>E. coli</em>, <em>Enterococcus</em>, antibiotic resistant fecal indicator bacteria (ARBs) tylosin resistant <em>Enterococcus</em>, tetracycline resistant <em>Enterococcus</em>, and antibiotic resistance genes (ARGs): <em>ermB</em>, <em>ermF</em> (macrolide), <em>tetA</em>, <em>tetM</em>, <em>tetO</em>, <em>tetW</em> (tetracycline), <em>sul1</em>, <em>sul2</em> (sulfonamide), <em>aadA2</em> (aminoglycoside), <em>vgaA</em>, and <em>vgaB</em> (pleuromutilin). Both the plate count and the ARG analyses showed seasonal trends. Plate counts were significantly greater during the growing season, while the ARGs were greater in the pre-planting and post-harvest seasons (Wilcoxon Rank-Sum Test p < 0.05). The <em>ermB</em> gene concentration was significantly correlated (p < 0.05) with <em>E. coli</em> and <em>Enterococcus</em> concentrations in 2017, suggesting a potential use of this ARG as an indicator of environmental AMR and human health risk. Flow rate was not a significant contributor to annual variations in bacteria and AMR indicators. Based on observed seasonal patterns, we concluded that manure application was the likely contributor to elevated ARG indicators observed in the BHL watershed, while the driver of elevated ARB indictors in the growing season can only be speculated. Understanding AMR export patterns in agricultural watersheds provides public health officials knowledge of seasonal periods of higher AMR load to recreational waters.</p>
dc.description.comments <p>This is a manuscript of an article published as Neher, Timothy P., Lanying Ma, Thomas B. Moorman, Adina Howe, and Michelle L. Soupir. "Seasonal variations in export of antibiotic resistance genes and bacteria in runoff from an agricultural watershed in Iowa." <em>Science of The Total Environment</em> (2020): 140224. DOI: <a href="https://doi.org/10.1016/j.scitotenv.2020.140224" target="_blank">10.1016/j.scitotenv.2020.140224</a>. </p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/abe_eng_pubs/1140/
dc.identifier.articleid 2425
dc.identifier.contextkey 18224415
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_pubs/1140
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/846
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/1140/2020_SoupirMichelle_SeasonalVariations.pdf|||Fri Jan 14 18:49:37 UTC 2022
dc.source.uri 10.1016/j.scitotenv.2020.140224
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.disciplines Environmental Monitoring
dc.subject.disciplines Water Resource Management
dc.subject.keywords Antimicrobial resistance
dc.subject.keywords Antibiotic resistance genes
dc.subject.keywords Water quality
dc.subject.keywords Fecal indicator bacteria
dc.subject.keywords Agriculture
dc.subject.keywords Manure
dc.title Seasonal variations in export of antibiotic resistance genes and bacteria in runoff from an agricultural watershed in Iowa
dc.type article
dc.type.genre article
dspace.entity.type Publication
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