Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves

Date
2022-04-15
Authors
Brito-Goulart, Débora
Beyi, Ashenafi Feyisa
Wu, Zuowei
Adiguzel, Mehmet Cemal
Schroeder, Anastasia
Singh, Kritika
Xu, Changyun
Ocal, Melda Meral
Dewell, Renee
Dewell, Grant
Plummer, Paul
Zhang, Qijing
Sahin, Orhan
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MDPI
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Brito-Goulart, Débora
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Veterinary Diagnostic and Production Animal MedicineVeterinary Microbiology and Preventive MedicineCenter for Food Security and Public Health
Abstract
Campylobacter is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) Campylobacter in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in C. jejuni, 30 commercial calves were divided into Group 1, Group 2, and Group 3 (n = 10), and were all inoculated orally with FQ-susceptible (FQ-S) C. jejuni; seven days later, Group 3 was challenged with transtracheal Mannheimia haemolytica, and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin. Rectal feces were collected to determine relative percentages of FQ-R Campylobacter via culture. Before oral inoculation with C. jejuni, 87% of calves were naturally colonized by FQ-R C. jejuni. Two days after the inoculation, FQ-R C. jejuni decreased substantially in the majority of calves. Within 24 h of danofloxacin injection, almost all C. jejuni populations shifted to an FQ-R phenotype in both FQ-treated groups, which was only transitory, as FQ-S strains became predominant during later periods. Genotyping indicated that the spike seen in FQ-R C. jejuni populations following the injection was due mainly to enrichment of preexisting FQ-R C. jejuni, rather than development of de novo FQ resistance in susceptible strains. These results provide important insights into the dynamic changes of FQ-resistant Campylobacter in cattle in response to FQ treatment.
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This article is published as Goulart, Debora Brito, Ashenafi Feyisa Beyi, Zuowei Wu, Mehmet Cemal Adiguzel, Anastasia Schroeder, Kritika Singh, Changyun Xu et al. "Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves." Antibiotics 11, no. 4 (2022): 531. DOI: 10.3390/antibiotics11040531. Copyright 2022 The Authors. Attribution 4.0 International (CC BY 4.0). Posted with permission.
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