Liver transcriptome response to hyperthermic stress in three distinct chicken lines

dc.contributor.author Lan, Xi
dc.contributor.author Hsieh, John
dc.contributor.author Lamont, Susan
dc.contributor.author Schmidt, Carl
dc.contributor.author Zhu, Qing
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2020-10-07T02:47:26.000
dc.date.accessioned 2021-02-24T21:11:51Z
dc.date.available 2021-02-24T21:11:51Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-11-22
dc.description.abstract <p><strong>Background</strong> High ambient temperatures cause stress in poultry, especially for broiler lines, which are genetically selected for rapid muscle growth. RNA-seq technology provides powerful insights into environmental response from a highly metabolic tissue, the liver. We investigated the effects of acute (3 h, 35 °C) and chronic (7d of 35 °C for 7 h/d) heat stress on the liver transcriptome of 3-week-old chicks of a heat-susceptible broiler line, a heat-resistant Fayoumi line, and their advanced intercross line (AIL).</p> <p><strong>Results</strong> Transcriptome sequencing of 48 male chickens using Illumina HiSeq 2500 technology yielded an average of 33.9 million, 100 base-pair, single-end reads per sample. There were 8 times more differentially expressed genes (DEGs) (FDR < 0.05) in broilers (n = 627) than Fayoumis (n = 78) when comparing the acute-heat samples to the control (25 °C) samples. Contrasting genetic lines under similar heat treatments, the highest number of DEGs appeared between Fayoumi and broiler lines. Principal component analysis of gene expression and analysis of the number of DEGs suggested that the AIL had a transcriptomic response more similar to the Fayoumi than the broiler line during acute heat stress. The number of DEGs also suggested that acute heat stress had greater impact on the broiler liver transcriptome than chronic heat stress. The angiopoietin-like 4 (ANGPTL4) gene was identified as differentially expressed among all 6 contrasts. Ingenuity Pathway Analysis (IPA) created a novel network that combines the heat shock protein family with immune response genes.</p> <p><strong>Conclusions</strong> This study extends our understanding of the liver transcriptome response to different heat exposure treatments in distinct genetic chicken lines and provides information necessary for breeding birds to be more resilient to the negative impacts of heat. The data strongly suggest ANGPTL4 as a candidate gene for improvement of heat tolerance in chickens.</p>
dc.description.comments <p>This article is published as Lan, Xi, John C.F. Hsieh, Carl J. Schmidt, Qing Zhu, and Susan J. Lamont. "Liver transcriptome response to hyperthermic stress in three distinct chicken lines." <em>BMC Genomics</em> 17 (2016): 955. DOI: <a href="https://doi.org/10.1186/s12864-016-3291-0" target="_blank">10.1186/s12864-016-3291-0</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/588/
dc.identifier.articleid 1585
dc.identifier.contextkey 19683483
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/588
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/93343
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/588/2016_LamontSusan_TissueExpression.pdf|||Sat Jan 15 01:02:32 UTC 2022
dc.source.uri 10.1186/s12864-016-3291-0
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords Host defence peptides (HDPs)
dc.subject.keywords Antimicrobial
dc.subject.keywords Chicken
dc.title Liver transcriptome response to hyperthermic stress in three distinct chicken lines
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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