Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids

dc.contributor.author Gomes Neto, Joao
dc.contributor.author Bower, Leslie
dc.contributor.author Erickson, Barbara
dc.contributor.author Wang, Chong
dc.contributor.author Raymond, Matthew
dc.contributor.author Strait, Erin
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2018-02-17T17:58:12.000
dc.date.accessioned 2020-07-07T05:13:35Z
dc.date.available 2020-07-07T05:13:35Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2015
dc.date.issued 2015-06-01
dc.description.abstract <p><em>Mycoplasma</em> (<em>M.</em>) <em>hyorhinis</em> and <em>M. hyosynoviae</em> are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect <em>M. hyorhinis</em> and <em>M. hyosynoviae</em> in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd surveillance. Two methods of genomic DNA extraction, automated versus manual, were used to compare diagnostic test performance. A wean-to-finish longitudinal study was also carried out to demonstrate the reproducibility of using pen-based oral fluids. Overall, pen-based oral and tonsillar fluids were more likely to be positive for both types of bacteria whereas only <em>M. hyorhinis</em> was detected in nasal fluids. DNA extraction protocols were shown to significantly influence test result. Although the initial detection time somewhat differed, both organisms were repeatedly detected in the longitudinal study. Overall, this study evaluated two qPCR methods for rapid and specific detection of either mycoplasma. Results from the present investigation can serve as a foundation for future studies to determine the prevalence of the two microorganisms, environmental load, and effectiveness of veterinary interventions for infection control.</p>
dc.description.comments <p>This article is from <em>Journal of Veterinary Science </em>16 (2015): 195, doi:<a href="http://dx.doi.org/%2010.4142/jvs.2015.16.2.195%20" target="_blank"> 10.4142/jvs.2015.16.2.195 </a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/68/
dc.identifier.articleid 1070
dc.identifier.contextkey 8740537
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/68
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92096
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/68/0-Journal_of_Veterinary_Science_permission.pdf|||Sat Jan 15 01:28:44 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/68/2015_Wang_QuantitativeReal.pdf|||Sat Jan 15 01:28:46 UTC 2022
dc.source.uri 10.4142/jvs.2015.16.2.195
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Other Veterinary Medicine
dc.subject.disciplines Veterinary Anatomy
dc.subject.disciplines Veterinary Physiology
dc.subject.keywords Mycoplasma hyorhinis
dc.subject.keywords Mycoplasma hyosynoviae
dc.subject.keywords real-time PCR
dc.subject.keywords swine
dc.title Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication b715071c-c3bd-419c-b021-0ac4702f346a
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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