Species-specific escape of Plasmodium sporozoites from oocysts of avian, rodent, and human malarial parasites

dc.contributor.author Orfano, Alessandra
dc.contributor.author Nacif-Pimenta, Rafaek
dc.contributor.author Smith, Ryan
dc.contributor.author Durate, Ana
dc.contributor.author Villegas, Luis
dc.contributor.author Rodrigues, Nilton
dc.contributor.author Pinto, Luciana
dc.contributor.author Campos, Keillen
dc.contributor.author Pinilla, Yudi
dc.contributor.author Chaves, Barbara
dc.contributor.author Barbosa Guerra, Maria
dc.contributor.author Monteiro, Wuelton
dc.contributor.author Smith, Ryan
dc.contributor.author Molina-Cruz, Alvaro
dc.contributor.author Lacerda, Marcus
dc.contributor.author Secundino, Nagila
dc.contributor.author Jacobs-Lorena, Marcelo
dc.contributor.author Barillas-Mury, Carolina
dc.contributor.author Pimenta, Paulo
dc.contributor.department Entomology
dc.date 2018-02-19T05:47:31.000
dc.date.accessioned 2020-06-30T02:24:11Z
dc.date.available 2020-06-30T02:24:11Z
dc.date.issued 2016-01-01
dc.description.abstract <p><h3>Background</h3> <p id="x-x-Par1">Malaria is transmitted when an infected mosquito delivers <em>Plasmodium</em> sporozoites into a vertebrate host. There are many species of <em>Plasmodium</em> and, in general, the infection is host-specific. For example<em>, Plasmodium gallinaceum</em> is an avian parasite, while <em>Plasmodium berghei</em> infects mice. These two parasites have been extensively used as experimental models of malaria transmission<em>. Plasmodium falciparum</em> and <em>Plasmodium vivax</em> are the most important agents of human malaria, a life-threatening disease of global importance. To complete their life cycle, <em>Plasmodium</em> parasites must traverse the mosquito midgut and form an oocyst that will divide continuously. Mature oocysts release thousands of sporozoites into the mosquito haemolymph that must reach the salivary gland to infect a new vertebrate host. The current understanding of the biology of oocyst formation and sporozoite release is mostly based on experimental infections with <em>P.</em> <em>berghei</em>, and the conclusions are generalized to other <em>Plasmodium</em> species that infect humans without further morphological analyses. <h3>Results</h3> <p id="x-x-Par2">Here, it is described the microanatomy of sporozoite escape from oocysts of four <em>Plasmodium</em> species: the two laboratory models, <em>P. gallinaceum</em> and <em>P. berghei</em>, and the two main species that cause malaria in humans, <em>P.</em> <em>viv</em>ax and <em>P. falciparum</em>. It was found that sporozoites have species-specific mechanisms of escape from the oocyst. The two model species of <em>Plasmodium</em> had a common mechanism, in which the oocyst wall breaks down before sporozoites emerge. In contrast, <em>P. vivax</em> and <em>P. falciparum</em> sporozoites show a dynamic escape mechanism from the oocyst via polarized propulsion. <h3>Conclusions</h3> <p id="x-x-Par3">This study demonstrated that <em>Plasmodium</em> species do not share a common mechanism of sporozoite escape, as previously thought, but show complex and species-specific mechanisms. In addition, the knowledge of this phenomenon in human <em>Plasmodium</em> can facilitate transmission-blocking studies and not those ones only based on the murine and avian models.</p>
dc.description.comments <p>Published as Orfano, Alessandra S., Rafael Nacif-Pimenta, Ana PM Duarte, Luis M. Villegas, Nilton B. Rodrigues, Luciana C. Pinto, Keillen MM Campos et al. "Species-specific escape of Plasmodium sporozoites from oocysts of avian, rodent, and human malarial parasites." Malaria journal 15, no. 1 (2016): 394. doi: <a href="http://dx.doi.org/10.1186/s12936-016-1451-y" target="_blank">10.1186/s12936-016-1451-y</a></p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ent_pubs/433/
dc.identifier.articleid 1435
dc.identifier.contextkey 11194973
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/433
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/24058
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/433/s12936_016_1451_y.pdf|||Sat Jan 15 00:15:54 UTC 2022
dc.source.uri 10.1186/s12936-016-1451-y
dc.subject.disciplines Entomology
dc.subject.keywords Sporozoites escape
dc.subject.keywords oocyst
dc.subject.keywords mosquito vector
dc.subject.keywords Plasmodium
dc.subject.keywords human
dc.subject.keywords murine
dc.subject.keywords avian
dc.title Species-specific escape of Plasmodium sporozoites from oocysts of avian, rodent, and human malarial parasites
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 2608f992-004b-43c0-a1bc-597c6b50b946
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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