The new genetic environment of cfr on plasmid pBS-02 in a Bacillus strain

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2011-05-01
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Zhang, Wan-Jiang
Wu, Cong-Ming
Wang, Yang
Shen, Zhang-Qi
Dai, Lei
Han, Jing
Foley, Steven
Shen, Jian-Zhong
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Zhang, Qijing
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Veterinary Microbiology and Preventive Medicine
Our faculty promote the understanding of causes of infectious disease in animals and the mechanisms by which diseases develop at the organismal, cellular and molecular levels. Veterinary microbiology also includes research on the interaction of pathogenic and symbiotic microbes with their hosts and the host response to infection.
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Abstract

Sir,

The gene cfr, encoding a 23S rRNA methyltransferase, confers resistance to five chemically unrelated antimicrobial classes, including phenicols, lincosamides, oxazolidinones, pleuromutilins and streptogramin A (PhLOPSA), and has been observed mainly in staphylococcal isolates over the past decade.1–3 Our previous study reported a cfr-carrying plasmid, pBS-01, in a novel strain (BS-01) of a Bacillus species isolated from a pig farm in China.4 In our routine surveillance study on antimicrobial resistance in farm animals in 2010, another florfenicol and chloramphenicol-resistant Bacillus species strain (named BS-02) from swine faeces was identified. Gram staining, sequence analysis of the 16S rRNA and API 50CH testing associated with the API 20E system (bioMérieux, France) showed that BS-02 had an identical profile to that of strain BS-01, which suggested that BS-02 also belongs to a novel Bacillus species. Despite their identical profiles, more than six PFGE band differences were observed between BS-01 and BS-02 (data not shown), indicating that they belong to different clonal types of the same species.

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This article is published as Zhang, Wan-Jiang, Cong-Ming Wu, Yang Wang, Zhang-Qi Shen, Lei Dai, Jing Han, Steven L. Foley, Jian-Zhong Shen, and Qijing Zhang. "The new genetic environment of cfr on plasmid pBS-02 in a Bacillus strain." Journal of antimicrobial chemotherapy 66, no. 5 (2011): 1174-1175. doi: 10.1093/jac/dkr037. Posted with permission.

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