The new genetic environment of cfr on plasmid pBS-02 in a Bacillus strain

Date
2011-05-01
Authors
Zhang, Wan-Jiang
Wu, Cong-Ming
Wang, Yang
Shen, Zhang-Qi
Dai, Lei
Han, Jing
Foley, Steven
Shen, Jian-Zhong
Zhang, Qijing
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Abstract

Sir,

The gene cfr, encoding a 23S rRNA methyltransferase, confers resistance to five chemically unrelated antimicrobial classes, including phenicols, lincosamides, oxazolidinones, pleuromutilins and streptogramin A (PhLOPSA), and has been observed mainly in staphylococcal isolates over the past decade.1–3 Our previous study reported a cfr-carrying plasmid, pBS-01, in a novel strain (BS-01) of a Bacillus species isolated from a pig farm in China.4 In our routine surveillance study on antimicrobial resistance in farm animals in 2010, another florfenicol and chloramphenicol-resistant Bacillus species strain (named BS-02) from swine faeces was identified. Gram staining, sequence analysis of the 16S rRNA and API 50CH testing associated with the API 20E system (bioMérieux, France) showed that BS-02 had an identical profile to that of strain BS-01, which suggested that BS-02 also belongs to a novel Bacillus species. Despite their identical profiles, more than six PFGE band differences were observed between BS-01 and BS-02 (data not shown), indicating that they belong to different clonal types of the same species.

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This article is published as Zhang, Wan-Jiang, Cong-Ming Wu, Yang Wang, Zhang-Qi Shen, Lei Dai, Jing Han, Steven L. Foley, Jian-Zhong Shen, and Qijing Zhang. "The new genetic environment of cfr on plasmid pBS-02 in a Bacillus strain." Journal of antimicrobial chemotherapy 66, no. 5 (2011): 1174-1175. doi: 10.1093/jac/dkr037. Posted with permission.

Keywords
florfenicol resistance, IS256, restriction-modification system
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