Evaluation and use of a serological assay for the detection of antibodies to Lawsonia intracellularis in swine

Date
2014-12-01
Authors
Magtoto, Ronaldo
Vegi, Anuradha
Wang, Chong
Johnson, John
Ramamoorthy, Sheela
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Wang, Chong
Person
Research Projects
Organizational Units
Journal Issue
Series
Abstract

Porcine proliferative enteropathy (PPE) is a common and economically important gastro-intestinal disease of swine caused by the intracellular bacterium, Lawsonia intracellularis. Conventional tests to detect antibody responses to L. intracellularisinclude the immuno-peroxidase monolayer assay (IPMA), immuno-fluorescent antibody test (IFAT) and a lipopolysaccharide ELISA (LPS-ELISA). These tests are not commercially available. Therefore, objective of this study is to evaluate the performance of a commercial L. intracellularis blocking ELISA. Performance of the commercial ELISA was compared to the IPMA and LPS-ELISA using serum from experimentally infected animals (N = 40). The prevalence of L. intracellularis sero-positive animals was assessed by comparing suspect and randomly selected sera (N = 394). The commercial ELISA, IPMA and a non-commercial lipopolysaccharide (LPS) LPS-ELISA showed a 95% correlation when tested using experimentally derived known status samples. When compared to the IPMA the sensitivity of the commercial ELISA was 91% while the specificity was 100%. Therefore, the diagnostic sensitivity and specificity of the commercial L. intracellularis ELISA was comparable to the LPS-ELISA and IPMA. A comparison of suspect and randomly selected field samples with the commercial ELISA indicated that L. intracellularis sero-positivity is widespread and does not correlate with possible disease status.

Description

This article is from International Journal of Veterinary Science and Medicine 2 (2014); 109, doi: 10.1016/j.ijvsm.2014.08.001. Posted with permission.

Keywords
Enzyme linked immunosorbent assay ELISA, Antibodies, Immuno-peroxidase monolayer assay, IPMA, Swine, Ileitis, Lawsonia intracellularis
Citation
DOI
Collections