Evaluation and use of a serological assay for the detection of antibodies to Lawsonia intracellularis in swine

dc.contributor.author Magtoto, Ronaldo
dc.contributor.author Vegi, Anuradha
dc.contributor.author Wang, Chong
dc.contributor.author Wang, Chong
dc.contributor.author Johnson, John
dc.contributor.author Ramamoorthy, Sheela
dc.contributor.department Statistics
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2018-02-17T17:40:25.000
dc.date.accessioned 2020-07-07T05:13:31Z
dc.date.available 2020-07-07T05:13:31Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-12-01
dc.description.abstract <p>Porcine proliferative enteropathy (PPE) is a common and economically important gastro-intestinal disease of swine caused by the intracellular bacterium, <em>Lawsonia intracellularis.</em> Conventional tests to detect antibody responses to <em>L. intracellularis</em>include the immuno-peroxidase monolayer assay (IPMA), immuno-fluorescent antibody test (IFAT) and a lipopolysaccharide ELISA (LPS-ELISA). These tests are not commercially available. Therefore, objective of this study is to evaluate the performance of a commercial <em>L. intracellularis</em> blocking ELISA. Performance of the commercial ELISA was compared to the IPMA and LPS-ELISA using serum from experimentally infected animals (<em>N</em> = 40). The prevalence of <em>L. intracellularis</em> sero-positive animals was assessed by comparing suspect and randomly selected sera (<em>N</em> = 394). The commercial ELISA, IPMA and a non-commercial lipopolysaccharide (LPS) LPS-ELISA showed a 95% correlation when tested using experimentally derived known status samples. When compared to the IPMA the sensitivity of the commercial ELISA was 91% while the specificity was 100%. Therefore, the diagnostic sensitivity and specificity of the commercial <em>L. intracellularis</em> ELISA was comparable to the LPS-ELISA and IPMA. A comparison of suspect and randomly selected field samples with the commercial ELISA indicated that <em>L. intracellularis</em> sero-positivity is widespread and does not correlate with possible disease status.</p>
dc.description.comments <p>This article is from<em> International Journal of Veterinary Science and Medicine </em>2 (2014); 109, doi: <a href="http://dx.doi.org/10.1016/j.ijvsm.2014.08.001" target="_blank">10.1016/j.ijvsm.2014.08.001</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/59/
dc.identifier.articleid 1051
dc.identifier.contextkey 8679297
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/59
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92087
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/59/2014_Wang_EvaluationUse.pdf|||Sat Jan 15 01:03:18 UTC 2022
dc.source.uri 10.1016/j.ijvsm.2014.08.001
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Statistical Methodology
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Physiology
dc.subject.keywords Enzyme linked immunosorbent assay ELISA
dc.subject.keywords Antibodies
dc.subject.keywords Immuno-peroxidase monolayer assay
dc.subject.keywords IPMA
dc.subject.keywords Swine
dc.subject.keywords Ileitis
dc.subject.keywords Lawsonia intracellularis
dc.title Evaluation and use of a serological assay for the detection of antibodies to Lawsonia intracellularis in swine
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication b715071c-c3bd-419c-b021-0ac4702f346a
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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