Functional Characterization of a Chicken Major Histocompatibility Complex Class II B Gene Promoter Chen, Yunfei Lillehoj, Hyun Lamont, Susan Hsu, Chung-Hsin Carpenter, Susan Lamont, Susan
dc.contributor.department Animal Science 2018-02-17T06:21:53.000 2020-06-29T23:39:02Z 2020-06-29T23:39:02Z 1997
dc.description.abstract <p>A 0.7 kilobase (kb) DNA fragment from the 5' flanking region of a chicken major histocompatibility complex (MHC) class II B gene was cloned into chloramphenicol acetyltransferase (CAT) reporter vectors and was transfected into a chicken macrophage cell line that expresses a low level of MHC class II antigens. Positive orientation-dependent promoter activity of the chicken DNA was evident in a reporter construct containing an SV40 enhancer. Deletion analysis of this 0.7 kb DNA fragment revealed a short fragment in the 3' end that was crucial for the promoter function and negative regulatory elements (NRE) located further upstream. The conserved MHC class II X and Y boxes did not have a significant effect on promoter activity. Sequence analysis of the 0.7 kb class II B gene upstream region suggests possible involvement of interferon (IFN), E twenty-six specific (ETS)-related proteins, and other factors in regulating this promoter. A chicken T-cell line culture supernatant increased surface expression of MHC class II antigens, as well as class II promoter activity, in this macrophage cell line. This first functional characterization of a chicken MHC class II B gene promoter will aid in understanding the regulatory mechanisms that control the expression of these genes.</p>
dc.description.comments <p>This article is from <em>Immunogenetics</em> 45 (1997): 242.</p>
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dc.identifier.articleid 1207
dc.identifier.contextkey 7855935
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dc.language.iso en
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dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Poultry or Avian Science
dc.title Functional Characterization of a Chicken Major Histocompatibility Complex Class II B Gene Promoter
dc.type article
dc.type.genre article
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relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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