Functional Characterization of a Chicken Major Histocompatibility Complex Class II B Gene Promoter

dc.contributor.author Chen, Yunfei
dc.contributor.author Lillehoj, Hyun
dc.contributor.author Lamont, Susan
dc.contributor.author Hsu, Chung-Hsin
dc.contributor.author Carpenter, Susan
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2018-02-17T06:21:53.000
dc.date.accessioned 2020-06-29T23:39:02Z
dc.date.available 2020-06-29T23:39:02Z
dc.date.issued 1997
dc.description.abstract <p>A 0.7 kilobase (kb) DNA fragment from the 5' flanking region of a chicken major histocompatibility complex (MHC) class II B gene was cloned into chloramphenicol acetyltransferase (CAT) reporter vectors and was transfected into a chicken macrophage cell line that expresses a low level of MHC class II antigens. Positive orientation-dependent promoter activity of the chicken DNA was evident in a reporter construct containing an SV40 enhancer. Deletion analysis of this 0.7 kb DNA fragment revealed a short fragment in the 3' end that was crucial for the promoter function and negative regulatory elements (NRE) located further upstream. The conserved MHC class II X and Y boxes did not have a significant effect on promoter activity. Sequence analysis of the 0.7 kb class II B gene upstream region suggests possible involvement of interferon (IFN), E twenty-six specific (ETS)-related proteins, and other factors in regulating this promoter. A chicken T-cell line culture supernatant increased surface expression of MHC class II antigens, as well as class II promoter activity, in this macrophage cell line. This first functional characterization of a chicken MHC class II B gene promoter will aid in understanding the regulatory mechanisms that control the expression of these genes.</p>
dc.description.comments <p>This article is from <em>Immunogenetics</em> 45 (1997): 242.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/211/
dc.identifier.articleid 1207
dc.identifier.contextkey 7855935
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/211
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9618
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/211/1997_Lamont_FunctionalCharacterization.pdf|||Fri Jan 14 22:34:47 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Poultry or Avian Science
dc.title Functional Characterization of a Chicken Major Histocompatibility Complex Class II B Gene Promoter
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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