The Fourier convolution-deconvolution method: its limitation and application

dc.contributor.author Gugliotta, Nicole
dc.contributor.department Chemistry
dc.date 2020-06-23T20:27:38.000
dc.date.accessioned 2020-06-30T08:15:32Z
dc.date.available 2020-06-30T08:15:32Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.issued 2006-01-01
dc.description.abstract <p>The ability to measure distances within a biomolecule is an important tool for determining structure and function. The electron paramagnetic resonance (EPR) Fourier Convolution-Deconvolution method of measuring distance was utilized to determine the distance between two cysteine mutated residues in the linker region of Sso1p in order to determine the conformation of this region during SNARE complex assembly. A 12 -14 Angstrom distance measurement suggests this region to alpha helical, with a small unstructured area, during complex formation. In addition the limits of distance measurements were tested using the continuous wave electron paramagnetic resonance (cwEPR) method of Fourier Convolution-Deconvolution. This method was unable to measure a known 32 Angstrom distance within the C-terminus region of the SNAP-25 protein.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/19409/
dc.identifier.articleid 20408
dc.identifier.contextkey 18210249
dc.identifier.doi https://doi.org/10.31274/rtd-20200622-58
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/19409
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/73416
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/19409/Gugliotta_ISU_2006_G84.pdf|||Fri Jan 14 21:56:28 UTC 2022
dc.subject.keywords Chemistry
dc.title The Fourier convolution-deconvolution method: its limitation and application
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
thesis.degree.discipline Chemistry
thesis.degree.level thesis
thesis.degree.name Master of Science
File
Original bundle
Now showing 1 - 1 of 1
Name:
Gugliotta_ISU_2006_G84.pdf
Size:
4.63 MB
Format:
Adobe Portable Document Format
Description: