The Fourier convolution-deconvolution method: its limitation and application Gugliotta, Nicole
dc.contributor.department Chemistry 2020-06-23T20:27:38.000 2020-06-30T08:15:32Z 2020-06-30T08:15:32Z Sun Jan 01 00:00:00 UTC 2006 2006-01-01
dc.description.abstract <p>The ability to measure distances within a biomolecule is an important tool for determining structure and function. The electron paramagnetic resonance (EPR) Fourier Convolution-Deconvolution method of measuring distance was utilized to determine the distance between two cysteine mutated residues in the linker region of Sso1p in order to determine the conformation of this region during SNARE complex assembly. A 12 -14 Angstrom distance measurement suggests this region to alpha helical, with a small unstructured area, during complex formation. In addition the limits of distance measurements were tested using the continuous wave electron paramagnetic resonance (cwEPR) method of Fourier Convolution-Deconvolution. This method was unable to measure a known 32 Angstrom distance within the C-terminus region of the SNAP-25 protein.</p>
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dc.identifier archive/
dc.identifier.articleid 20408
dc.identifier.contextkey 18210249
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/19409
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 21:56:28 UTC 2022
dc.subject.keywords Chemistry
dc.title The Fourier convolution-deconvolution method: its limitation and application
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11 Chemistry thesis Master of Science
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