Genetic and biochemical differences in populations bred for extremes in maize grain methionine concentration

dc.contributor.author Newell, Mark
dc.contributor.author Vogel, Karla
dc.contributor.author Scott, Marvin
dc.contributor.author Adams, Marie
dc.contributor.author Aydin, Nevzat
dc.contributor.author Bodnar, Anastasia
dc.contributor.author Ali, Muhammad
dc.contributor.author Moran Lauter, Adrienne
dc.contributor.author Scott, M Paul
dc.contributor.department Agronomy
dc.contributor.department Genetics
dc.date 2018-02-18T14:46:33.000
dc.date.accessioned 2020-06-29T23:03:00Z
dc.date.available 2020-06-29T23:03:00Z
dc.date.issued 2014-01-01
dc.description.abstract <p><h3>Background</h3></p> <p>Methionine is an important nutrient in animal feed and several approaches have been developed to increase methionine concentration in maize (<em>Zea mays</em> L.) grain. One approach is through traditional breeding using recurrent selection. Using divergent selection, genetically related populations with extreme differences in grain methionine content were produced. In order to better understand the molecular mechanisms controlling grain methionine content, we examined seed proteins, transcript levels of candidate genes, and genotypes of these populations. <h3>Results</h3></p> <p>Two populations were selected for high or low methionine concentration for eight generations and 40 and 56% differences between the high and low populations in grain methionine concentration were observed. Mean values between the high and low methionine populations differed by greater than 1.5 standard deviations in some cycles of selection. Other amino acids and total protein concentration exhibited much smaller changes. In an effort to understand the molecular mechanisms that contribute to these differences, we compared transcript levels of candidate genes encoding high methionine seed storage proteins involved in sulfur assimilation or methionine biosynthesis. In combination, we also explored the genetic mechanisms at the SNP level through implementation of an association analysis. Significant differences in methionine-rich seed storage protein genes were observed in comparisons of high and low methionine populations, while transcripts of seed storage proteins lacking high levels of methionine were unchanged. Seed storage protein levels were consistent with transcript levels. Two genes involved in sulfur assimilation, <em>Cys2</em> and <em>CgS1</em> showed substantial differences in allele frequencies when two selected populations were compared to the starting populations. Major genes identified across cycles of selection by a high-stringency association analysis included <em>dzs18</em>, <em>wx</em>, <em>dzs10</em>, and <em>zp27</em>. <h3>Conclusions</h3></p> <p>We hypothesize that transcriptional changes alter sink strength by altering the levels of methionine-rich seed storage proteins. To meet the altered need for sulfur, a cysteine-rich seed storage protein is altered while sulfur assimilation and methionine biosynthesis throughput is changed by selection for certain alleles of <em>Cys2</em> and <em>CgS1</em>.</p>
dc.description.comments <p>This article is published as Newell, Mark A., Karla E. Vogel, Marie Adams, Nevzat Aydin, Anastasia L. Bodnar, Muhammad Ali, Adrienne N. Moran Lauter, and M. Paul Scott. "Genetic and biochemical differences in populations bred for extremes in maize grain methionine concentration." <em>BMC plant biology</em> 14, no. 1 (2014): 49, doi: <a href="http://dx.doi.org/10.1186/1471-2229-14-49" target="_blank">10.1186/1471-2229-14-49</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/156/
dc.identifier.articleid 1147
dc.identifier.contextkey 10423619
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/156
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4484
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/156/2014_Scott_GeneticBiochemical.pdf|||Fri Jan 14 20:43:40 UTC 2022
dc.source.uri 10.1186/1471-2229-14-49
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Methionine
dc.subject.keywords Breeding
dc.subject.keywords Association mapping
dc.subject.keywords Sulfur assimilation
dc.subject.keywords Storage proteins
dc.title Genetic and biochemical differences in populations bred for extremes in maize grain methionine concentration
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 97acee5f-1291-4c27-8929-a8e1617c411d
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
File
Original bundle
Now showing 1 - 1 of 1
Name:
2014_Scott_GeneticBiochemical.pdf
Size:
1.35 MB
Format:
Adobe Portable Document Format
Description:
Collections