IRE1B degrades RNAs encoding proteins that interfere with the induction of autophagy by ER stress in Arabidopsis thaliana

dc.contributor.author Bao, Yan
dc.contributor.author Pu, Yunting
dc.contributor.author Yu, Xiang
dc.contributor.author Gregory, Brian
dc.contributor.author Bassham, Diane
dc.contributor.author Srivastava, Renu
dc.contributor.author Howell, Stephen
dc.contributor.author Bassham, Diane
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Genetics and Genomics
dc.contributor.department Plant Sciences Institute
dc.date 2019-05-17T22:27:42.000
dc.date.accessioned 2020-06-30T04:01:58Z
dc.date.available 2020-06-30T04:01:58Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2018
dc.date.embargo 2019-08-17
dc.date.issued 2018-08-17
dc.description.abstract <p>Macroautophagy/autophagy is a conserved process in eukaryotes that contributes to cell survival in response to stress. Previously, we found that endoplasmic reticulum (ER) stress induces autophagy in plants via a pathway dependent upon AT5G24360/IRE1B (INOSITOL REQUIRING 1–1), an ER membrane-anchored factor involved in the splicing of <em>AT1G42990</em>/<em>BZIP60</em> (basic leucine zipper protein 60) mRNA. IRE1B is a dual protein kinase and ribonuclease, and here we determined the involvement of the protein kinase catalytic domain, nucleotide binding and RNase domains of IRE1B in activating autophagy. We found that the nucleotide binding and RNase activity of IRE1B, but not its protein kinase activity or splicing target <em>BZIP60</em>, are required for ER stress-mediated autophagy. Upon ER stress, the RNase activity of IRE1B engages in regulated IRE1-dependent decay of messenger RNA (RIDD), in which mRNAs of secreted proteins are degraded by IRE1 upon ER stress. Twelve genes most highly targeted by RIDD were tested for their role in inhibiting ER stress-induced autophagy, and 3 of their encoded proteins, AT1G66270/BGLU21 (β-glucosidase 21), AT2G16005/ROSY1/ML (MD2-related lipid recognition protein) and AT5G01870/PR-14 (pathogenesis-related protein 14), were found to inhibit autophagy upon overexpression. From these findings, IRE1B is posited to be a ‘licensing factor’ linking ER stress to autophagy by degrading the RNA transcripts of factors that interfere with the induction of autophagy.</p>
dc.description.comments <p>This is a manuscript of an article published as Bao, Yan, Yunting Pu, Xiang Yu, Brian D. Gregory, Renu Srivastava, Stephen H. Howell, and Diane C. Bassham. "IRE1B degrades RNAs encoding proteins that interfere with the induction of autophagy by ER stress in Arabidopsis thaliana." <em>Autophagy</em> 14, no. 9 (2018): 1562-1573. doi: <a href="https://doi.org/10.1080/15548627.2018.1462426">10.1080/15548627.2018.1462426</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/218/
dc.identifier.articleid 1222
dc.identifier.contextkey 14179929
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/218
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37892
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/218/2018_Bassham_DegradesEncodingManuscript.pdf|||Fri Jan 14 22:39:10 UTC 2022
dc.source.uri 10.1080/15548627.2018.1462426
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Genetics
dc.subject.disciplines Plant Biology
dc.subject.keywords Arabidopsis
dc.subject.keywords autophagosome
dc.subject.keywords autophagy
dc.subject.keywords ER stress
dc.subject.keywords IRE1
dc.subject.keywords mRNA degradation
dc.subject.keywords RIDD
dc.title IRE1B degrades RNAs encoding proteins that interfere with the induction of autophagy by ER stress in Arabidopsis thaliana
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 2d25a522-f3af-4bb3-9221-363ff3668fe0
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
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