Structural basis of DNA recognition of the Campylobacter jejuni CosR regulator

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Zhang, Zhemin
Yan, Yuqi
Pang, Jinji
Dai, Lei
Yu, Edward W.
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American Society for Microbiology (ASM)
Zhang, Qijing
Distinguished Professor
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Veterinary Microbiology and Preventive Medicine
Our faculty promote the understanding of causes of infectious disease in animals and the mechanisms by which diseases develop at the organismal, cellular and molecular levels. Veterinary microbiology also includes research on the interaction of pathogenic and symbiotic microbes with their hosts and the host response to infection.
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Campylobacter jejuni is a foodborne pathogen commonly found in the intestinal tracts of animals. This pathogen is a leading cause of gastroenteritis in humans. Besides its highly infectious nature, C. jejuni is increasingly resistant to a number of clinically administrated antibiotics. As a consequence, the Centers for Disease Control and Prevention has designated antibiotic-resistant Campylobacter as a serious antibiotic resistance threat in the United States. The C. jejuni CosR regulator is essential to the viability of this bacterium and is responsible for regulating the expression of a number of oxidative stress defense enzymes. Importantly, it also modulates the expression of the CmeABC multidrug efflux system, the most predominant and clinically important system in C. jejuni that mediates resistance to multiple antimicrobials. Here, we report structures of apo-CosR and CosR bound with a 21 bp DNA sequence located at the cmeABC promotor region using both single-particle cryo-electron microscopy and X-ray crystallography. These structures allow us to propose a novel mechanism for CosR regulation that involves a long-distance conformational coupling and rearrangement of the secondary structural elements of the regulator to bind target DNA.
This article is published as Zhang, Zhemin, Yuqi Yan, Jinji Pang, Lei Dai, Qijing Zhang, and Edward W. Yu. "Structural basis of DNA recognition of the Campylobacter jejuni CosR regulator." Mbio (2024): e03430-23. doi: Copyright © 2024 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.