Optical Imaging of the Nanoscale Structure and Dynamics of Biological Membranes

dc.contributor.author Wijesooriya, Chamari
dc.contributor.author Nyamekye, Charles
dc.contributor.author Smith, Emily
dc.contributor.author Smith, Emily
dc.contributor.department Ames National Laboratory
dc.contributor.department Chemistry
dc.date 2020-04-10T01:18:54.000
dc.date.accessioned 2020-06-30T01:17:37Z
dc.date.available 2020-06-30T01:17:37Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2018
dc.date.issued 2018-10-19
dc.description.abstract <p>Biological membranes serve as the fundamental unit of life, allowing the compartmentalization of cellular contents into subunits with specific functions. The bilayer structure, consisting of lipids, proteins, small molecules, and sugars, also serves many other complex functions in addition to maintaining the relative stability of the inner compartments. Signal transduction, regulation of solute exchange, active transport, and energy transduction through ion gradients all take place at biological membranes, primarily with the assistance of membrane proteins. For these functions, membrane structure is often critical. The fluid-mosaic model introduced by Singer and Nicolson in 1972 evokes the dynamic and fluid nature of biological membranes.<a>(1)</a> According to this model, integral and peripheral proteins are oriented in a viscous phospholipid bilayer. Both proteins and lipids can diffuse laterally through the two-dimensional structure. Modern experimental evidence has shown, however, that the structure of the membrane is considerably more complex; various domains in the biological membranes, such as lipid rafts and confinement regions, form a more complicated molecular organization. The proper organization and dynamics of the membrane components are critical for the function of the entire cell. For example, cell signaling is often initiated at biological membranes and requires receptors to diffuse and assemble into complexes and clusters, and the resulting downstream events have consequences throughout the cell. Revealing the molecular level details of these signaling events is the foundation to understanding numerous unsolved questions regarding cellular life.</p>
dc.description.comments <p>This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in <em>Analytical Chemistry</em>, copyright © American Chemical Society after peer review. To access the final edited and published work see DOI:<a href="https://doi.org/10.1021/acs.analchem.8b04755" target="_blank">10.1021/acs.analchem.8b04755</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/chem_pubs/1228/
dc.identifier.articleid 2232
dc.identifier.contextkey 17326912
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath chem_pubs/1228
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/14540
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/chem_pubs/1228/2018_SmithEmily_OpticalImaging.pdf|||Fri Jan 14 19:17:08 UTC 2022
dc.source.uri 10.1021/acs.analchem.8b04755
dc.subject.disciplines Analytical Chemistry
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Cancer Biology
dc.title Optical Imaging of the Nanoscale Structure and Dynamics of Biological Membranes
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
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