Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis

dc.contributor.author Jane, Jay-Lin
dc.contributor.author Yoo, Sang-Ho
dc.contributor.author Lee, Byung-Hoo
dc.contributor.author Moon, Youyoun
dc.contributor.author Spalding, Martin
dc.contributor.author Spalding, Martin
dc.contributor.author Jane, Jay-Lin
dc.contributor.department Food Science and Human Nutrition
dc.date 2018-02-19T03:47:05.000
dc.date.accessioned 2020-06-30T04:00:05Z
dc.date.available 2020-06-30T04:00:05Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-03-17
dc.description.abstract <p><em>Synechocystis</em> sp. PCC6803 belongs to cyanobacteria which carry out photosynthesis and has recently become of interest due to the evolutionary link between bacteria and plant species. Similar to other bacteria, the primary carbohydrate storage source of <em>Synechocystis</em> sp. PCC6803 is glycogen. While most bacteria are not known to have any isoforms of glycogen synthase, analysis of the genomic DNA sequence of <em>Synechocystis</em> sp. PCC6803 predicts that this strain encodes two isoforms of glycogen synthase (GS) for synthesizing glycogen structure. To examine the functions of the putative GS genes, each gene (<em>sll</em>1393 or <em>sll</em>0945) was disrupted by double cross-over homologous recombination. Zymogram analysis of the two GS disruption mutants allowed the identification of a protein band corresponding to each GS isoform. Results showed that two GS isoforms (GSI and GSII) are present in <em>Synechocystis</em> sp. PCC6803, and both are involved in glycogen biosynthesis with different elongation properties: GSI is processive and GSII is distributive. Total GS activities in the mutant strains were not affected and were compensated by the remaining isoform. Analysis of the branch-structure of glycogen revealed that the <em>sll</em>1393− mutant (GSI−) produced glycogen containing more intermediate-length chains (DP 8–18) at the expense of shorter and longer chains compared with the wild-type strain. The <em>sll</em>0945− mutant (GSII−) produced glycogen similar to the wild-type, with only a slightly higher proportion of short chains (DP 4–11). The current study suggests that GS isoforms in <em>Synechocystis</em> sp. PCC6803 have different elongation specificities in the biosynthesis of glycogen, combined with ADP-glucose pyrophosphorylase and glycogen branching enzyme.</p>
dc.description.comments <p>This is an article published as Yoo S-H, Lee B-H, Moon Y, Spalding MH, Jane J-l (2014) Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis. PLoS ONE 9(3): e91524. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0091524" target="_blank">10.1371/journal.pone.0091524</a>. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/fshn_hs_pubs/18/
dc.identifier.articleid 1018
dc.identifier.contextkey 11093929
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath fshn_hs_pubs/18
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37627
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/fshn_hs_pubs/18/2014_LeeJ_GlycogenSynthaseIsoforms.PDF|||Fri Jan 14 21:34:26 UTC 2022
dc.source.uri 10.1371/journal.pone.0091524
dc.subject.disciplines Food Chemistry
dc.subject.disciplines Food Processing
dc.subject.disciplines Food Science
dc.subject.disciplines Human and Clinical Nutrition
dc.subject.disciplines Molecular, Genetic, and Biochemical Nutrition
dc.title Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 7e504628-4633-4f5e-b092-a2ac15297649
relation.isAuthorOfPublication f69ccff8-25fa-4e13-b440-3c3d2ab9353a
relation.isOrgUnitOfPublication 95fe1086-c07b-408b-a017-f17053e4bfbf
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