Identification of RoCYP01 (CYP716A155) enables construction of engineered yeast for high-yield production of betulinic acid

dc.contributor.author Huang, Jiajian
dc.contributor.author Zha, Wenlong
dc.contributor.author Peters, Reuben
dc.contributor.author An, Tianyue
dc.contributor.author Dong, Hua
dc.contributor.author Huang, Ying
dc.contributor.author Wang, Dong
dc.contributor.author Yu, Rongmin
dc.contributor.author Duan, Lixin
dc.contributor.author Zhang, Xueli
dc.contributor.author Peters, Reuben
dc.contributor.author Dai, Zhubo
dc.contributor.author Zi, Jiachen
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2019-12-09T23:03:05.000
dc.date.accessioned 2020-06-29T23:46:47Z
dc.date.available 2020-06-29T23:46:47Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.embargo 2020-07-15
dc.date.issued 2019-07-15
dc.description.abstract <p>Betulinic acid (BA) and its derivatives possess potent pharmacological activity against cancer and HIV. As with many phytochemicals, access to BA is limited by the requirement for laborious extraction from plant biomass where it is found in low amounts. This might be alleviated by metabolically engineering production of BA into an industrially relevant microbe such as <em>Saccharomyces cerevisiae</em> (yeast), which requires complete elucidation of the corresponding biosynthetic pathway. However, while cytochrome P450 enzymes (CYPs) that can oxidize lupeol into BA have been previously identified from the CYP716A subfamily, these generally do not seem to be specific to such biosynthesis and, in any case, have not been shown to enable high-yielding metabolic engineering. Here RoCYP01 (CYP716A155) was identified from the BA-producing plant <em>Rosmarinus officinalis</em> (rosemary) and demonstrated to effectively convert lupeol into BA, with strong correlation of its expression and BA accumulation. This was further utilized to construct a yeast strain that yields > 1 g/L of BA, providing a viable route for biotechnological production of this valuable triterpenoid.</p>
dc.description.comments <p>This is a manuscript of an article published as Huang, Jiajian, Wenlong Zha, Tianyue An, Hua Dong, Ying Huang, Dong Wang, Rongmin Yu et al. "Identification of RoCYP01 (CYP716A155) enables construction of engineered yeast for high-yield production of betulinic acid." <em>Applied microbiology and biotechnology</em> (2019). doi: <a href="http://dx.doi.org/10.1007/s00253-019-10004-z" target="_blank">10.1007/s00253-019-10004-z</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/252/
dc.identifier.articleid 1260
dc.identifier.contextkey 15009045
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/252
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/10724
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/252/2019_Peters_IdentificationEnablesManuscript.pdf|||Fri Jan 14 22:57:48 UTC 2022
dc.source.uri 10.1007/s00253-019-10004-z
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Biotechnology
dc.subject.disciplines Molecular Biology
dc.subject.keywords betulinic acid
dc.subject.keywords cytochrome P450
dc.subject.keywords synthetic biology
dc.subject.keywords yeast Introduction
dc.title Identification of RoCYP01 (CYP716A155) enables construction of engineered yeast for high-yield production of betulinic acid
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 498a24ec-81d7-4bee-b145-323d38e7a392
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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