Characterization of DNA Sequences that Confer Complement Resistance in Leishmania chagasi

Date
2008-12-01
Authors
Dahlin-Laborde, Rebecca
Scolaro, Eric
Romine, Nathan
Ramer-Tait, Amanda
Lei, Soi Meng
Beetham, Jeffrey
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Entomology
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Abstract

Serial passage of axenically cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into high-passage (constitutively CML-sensitive) L. chagasi promastigotes; (2) transformants were screened for acquisition of CML-resistance; (3) multiple cosmid-transfectants exhibited partial CML resistance; and (4) the sequence for one of the cosmids (Cosmid 51) was determined. This report extends the analysis of Cosmid 51, and identifies by deletion analysis a subregion of the cosmid insert that is critical to the CML-resistance phenotype of Cosmid 51 transformants. We also report the sequence determination and initial CML-resistance activity of another cosmid that also confers partial resistance to CML.

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This is a manuscript of an article from Annals of the New York Academy of Sciences 1149 (2008): 347, doi:10.1196/annals.1428.099. Posted with permission.

Keywords
Leishmania, Leishmania chagasi, protozoa, complement, immunity
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