Characterization of DNA Sequences that Confer Complement Resistance in Leishmania chagasi

dc.contributor.author Dahlin-Laborde, Rebecca
dc.contributor.author Scolaro, Eric
dc.contributor.author Beetham, Jeffrey
dc.contributor.author Romine, Nathan
dc.contributor.author Ramer-Tait, Amanda
dc.contributor.author Lei, Soi Meng
dc.contributor.author Beetham, Jeffrey
dc.contributor.department Entomology
dc.date 2018-02-15T02:30:07.000
dc.date.accessioned 2020-06-30T02:22:57Z
dc.date.available 2020-06-30T02:22:57Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2008
dc.date.embargo 2008-12-01
dc.date.issued 2008-12-01
dc.description.abstract <p>Serial passage of axenically cultured <em>Leishmania chagasi</em> promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant <em>L. chagasi</em> promastigotes was transfected into high-passage (constitutively CML-sensitive) <em>L. chagasi</em> promastigotes; (2) transformants were screened for acquisition of CML-resistance; (3) multiple cosmid-transfectants exhibited partial CML resistance; and (4) the sequence for one of the cosmids (Cosmid 51) was determined. This report extends the analysis of Cosmid 51, and identifies by deletion analysis a subregion of the cosmid insert that is critical to the CML-resistance phenotype of Cosmid 51 transformants. We also report the sequence determination and initial CML-resistance activity of another cosmid that also confers partial resistance to CML.</p>
dc.description.comments <p>This is a manuscript of an article from <em>Annals of the New York Academy of Sciences</em> 1149 (2008): 347, doi:<a href="http://dx.doi.org/10.1196/annals.1428.099" target="_blank">10.1196/annals</a>.1428.099. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ent_pubs/266/
dc.identifier.articleid 1266
dc.identifier.contextkey 6226199
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/266
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/23880
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/266/2008_Beetham_CharacterizationDNA.pdf|||Fri Jan 14 23:03:29 UTC 2022
dc.source.uri 10.1196/annals.1428.099
dc.subject.disciplines Entomology
dc.subject.disciplines Immunology of Infectious Disease
dc.subject.keywords Leishmania
dc.subject.keywords Leishmania chagasi
dc.subject.keywords protozoa
dc.subject.keywords complement
dc.subject.keywords immunity
dc.title Characterization of DNA Sequences that Confer Complement Resistance in Leishmania chagasi
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1505fb83-30dc-4128-b6d9-29b02ed91f6b
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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