Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

dc.contributor.author Kautharapu, Kumar
dc.contributor.author Jarboe, Laura
dc.contributor.author Rathmacher, John
dc.contributor.author Jarboe, Laura
dc.contributor.department Chemical and Biological Engineering
dc.date 2018-02-15T16:49:04.000
dc.date.accessioned 2020-06-30T01:08:11Z
dc.date.available 2020-06-30T01:08:11Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2012
dc.date.embargo 2014-12-23
dc.date.issued 2013-01-01
dc.description.abstract <p>The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental carbon, nitrogen and salts, growth temperature and media composition and pH on DHA and biomass production and the fatty acid profile. The addition of supplemental nitrogen significantly increased the overall DHA titer via an increase in biomass production. Supplemental glucose or glycerol increased biomass production, but decreased the amount of DHA per biomass, resulting in no net change in the DHA titer. Acidification of the baseline media pH to 6.0 increased DHA per biomass. Changes in growth temperature or provision of supplemental sodium or magnesium chloride did not increase DHA titer. This organism was also shown to grow on defined minimal media. For both media types, glycerol enabled more DHA production per biomass than glucose. Combination of these growth findings into marine broth supplemented with glycerol, yeast extract, and tryptone at pH 6.0 resulted in a final titer of 82 ± 5 mg/L, a nearly eightfold increase relative to the titer of 11 ± 1 mg/L seen in the unsupplemented marine broth. The relative distribution of other fatty acids was relatively robust to growth condition, but the presence of glycerol resulted in a significant increase in myristic acid (C14:0) and decrease in palmitic acid (C16:0). In summary, DHA production by M. marina MP-1 can be increased more than fivefold by changing the growth media. Metabolic engineering of this organism to increase the amount of DHA produced per biomass could result in additional increases in titer.</p>
dc.description.comments <p>This is a manuscript of an article from <em>Applied Microbiology and Biotechnology</em> 97 (2013):2859, doi: <a href="http://dx.doi.org/10.1007/s00253-012-4529-7" target="_blank">10.1007/s00253-012-4529-7</a>. Posted with permission. The final publication is available at Springer -Verlag Berlin Heidelberg via http://dx.doi.org/10.1007/s00253-012-4529-7.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/174/
dc.identifier.articleid 1177
dc.identifier.contextkey 6488275
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/174
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13264
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/174/2013_JarboeLR_GrowthConditionOptimization_manuscript.pdf|||Fri Jan 14 21:22:03 UTC 2022
dc.source.uri 10.1007/s00253-012-4529-7
dc.subject.disciplines Biochemical and Biomolecular Engineering
dc.subject.disciplines Biological Engineering
dc.subject.disciplines Chemical Engineering
dc.subject.keywords marine broth
dc.subject.keywords membrane fluidity
dc.subject.keywords moritella marina MP-1
dc.subject.keywords polyketide
dc.subject.keywords polyunsaturated fatty acids
dc.title Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6113d68a-37ba-4092-8ed5-44d66391fae4
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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