Variation in seed dormancy in Echinochloa and the development of a standard protocol for germination testing

dc.contributor.author Kovach, David
dc.contributor.author Widrlechner, Mark
dc.contributor.author Brenner, David
dc.contributor.department North Central Regional Plant Introduction Station
dc.date 2018-02-13T05:26:42.000
dc.date.accessioned 2020-06-30T06:10:38Z
dc.date.available 2020-06-30T06:10:38Z
dc.date.embargo 2013-02-02
dc.date.issued 2010-10-01
dc.description.abstract <p>The North Central Regional Plant Introduction Station maintains more than 300 accessions of <em>Echinochloa</em> representing 15 species from a diverse cross-section of nations and growing conditions from around the world. With such a diverse collection, no single germination-testing protocol was adequate for accurately assessing their viability. By manipulating light conditions, we determined that some accessions were light-requiring and others were dark-requiring. However, no pattern was found for this response based on taxonomy or improvement status. Most accessions tested showed optimal germination when tests were conducted between 25 to 30°C, but both positive and negative photoblastic responses were sometimes expressed, even at lower temperatures. A sequential treatment of darkness followed by light revealed that skotodormancy (dormancy caused by darkness) was being induced in light-requiring seeds. Similarly, a sequential treatment with light followed by darkness revealed that photodormancy (dormancy caused by light) was being induced in dark-requiring seeds. Thus, without prior knowledge of the light requirements of a particular accession, we conclude that a side-by-side germination test where two replicates receive periodic light (12 hours at 30°C / 12 hours dark at 20°C) and the other two are tested in darkness (12 hours at 30°C / 12 hours at 20°C) is best for <em>Echinochloa</em>. This approach is recommended for germplasm centers, seed-testing laboratories, and others working with genetically and geographically diverse <em>Echinochloa</em> seed lots.</p>
dc.description.comments <p>This article is from <em>Seed Science and Technology </em>38, no. 3 (October 2010): 559–571.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ncrpis_pubs/17/
dc.identifier.articleid 1017
dc.identifier.contextkey 3644554
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ncrpis_pubs/17
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/55994
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ncrpis_pubs/17/Widrlechner_2010_VariationSeedDormancy.pdf|||Fri Jan 14 21:09:10 UTC 2022
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Horticulture
dc.subject.disciplines Plant Sciences
dc.title Variation in seed dormancy in Echinochloa and the development of a standard protocol for germination testing
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 8dcf3b33-db2f-46f8-8b80-20844d33fa84
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