Lachnospira pectinoschiza sp. nov., an Anaerobic Pectinophile from the Pig Intestine

dc.contributor.author Cornick, Nancy
dc.contributor.author Cornick, Nancy
dc.contributor.author Jensen, N. S.
dc.contributor.author Stahl, D. A.
dc.contributor.author Hartman, P. A.
dc.contributor.author Allison, M. J.
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-17T09:55:54.000
dc.date.accessioned 2020-07-07T05:14:19Z
dc.date.available 2020-07-07T05:14:19Z
dc.date.issued 1994
dc.description.abstract <p>Pectinophiles are bacteria that utilize pectin and only a few related compounds as substrates. Obligately anaerobic pectinophiles have been isolated from the intestinal tracts and gingivae of humans and from the rumina of cattle. We isolated three strains of pectinophilic bacteria from colonic contents of pigs but were unable to isolate pectinophiles from the rumen contents of four sheep, even when the animals were fed a high-pectin diet. The pectinophiles isolated from pigs were strictly anaerobic, motile, gram-positive rods (0.36 to 0.56 by 2.4 to 3.1 μm). Pectin, polygalacturonic acid, and gluconate were the only substrates that supported rapid growth. All three strains grew slowly on either lactose or cellobiose and fermented fructose after a lag of several days. Pectin was degraded by means of an extracellular pectin methylesterase and a Ca2+-dependent exopectate lyase. A comparison of the 16S rRNA sequences of these isolates with the 16S rRNA sequences of other gram-positive bacteria revealed a specific relationship with Lachnospira multipara (level of similarity, 94%). The Gram reaction, formation of spore-like structures, and the utilization of lactose and cellobiose differentiated the pig isolates from previously described pectinophiles. The pig isolates represent a previously undescribed species of the genus Lachnospira, for which we propose the name Lachnospira pectinoschiza.</p>
dc.description.comments <p>This article is from <em>International Journal of Systematic Microbiology</em> 44 (1994): 84, doi:<a href="http://dx.doi.org/10.1099/00207713-44-1-87" target="_blank">10.1099/00207713-44-1-87</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/105/
dc.identifier.articleid 1096
dc.identifier.contextkey 7982512
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/105
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92206
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/105/1994_Cornick_LachnospiraPectinoschiza.pdf|||Fri Jan 14 18:22:10 UTC 2022
dc.source.uri 10.1099/00207713-44-1-87
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Medicine
dc.title Lachnospira pectinoschiza sp. nov., an Anaerobic Pectinophile from the Pig Intestine
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication a89b8fad-4329-4bb1-bbcd-c1d3b324f0f7
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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