Transgenic maize endosperm containing a milk protein has improved amino acid balance

dc.contributor.author Bicar, Earl
dc.contributor.author Woodman-Clikeman, Wendy
dc.contributor.author Sangtong, Varaporn
dc.contributor.author Scott, Marvin
dc.contributor.author Peterson, Joan
dc.contributor.author Yang, S.
dc.contributor.author Lee, Michael
dc.contributor.author Scott, Marvin
dc.contributor.department Food Science and Human Nutrition
dc.contributor.department Agronomy
dc.contributor.department Genetics
dc.date 2018-02-17T13:45:05.000
dc.date.accessioned 2020-06-29T23:07:02Z
dc.date.available 2020-06-29T23:07:02Z
dc.date.issued 2008-02-01
dc.description.abstract <p>In order to meet the protein nutrition needs of the world population, greater reliance on plant protein sources will become necessary. The amino acid balance of most plant protein sources does not match the nutritional requirements of monogastric animals, limiting their nutritional value. In cereals, the essential amino acid lysine is deficient. Maize is a major component of human and animal diets worldwide and especially where sources of plant protein are in critical need such as sub-Saharan Africa. To improve the amino acid balance of maize, we developed transgenic maize lines that produce the milk protein α-lactalbumin in the endosperm. Lines in which the transgene was inherited as a single dominant genetic locus were identified. Sibling kernels with or without the transgene were compared to determine the effect of the transgene on kernel traits in lines selected for their high content of α-lactalbumin. Total protein content in endosperm from transgene positive kernels was not significantly different from total protein content in endosperm from transgene negative kernels in three out of four comparisons, whereas the lysine content of the lines examined was 29–47% greater in endosperm from transgene positive kernels. The content of some other amino acids was changed to a lesser extent. Taken together, these changes resulted in the transgenic endosperms having an improved amino acid balance relative to non-transgenic endosperms produced on the same ear. Kernel appearance, weight, density and zein content did not exhibit substantial differences in kernels expressing the transgene when compared to non-expressing siblings. Assessment of the antigenicity and impacts on animal health will be required in order to determine the overall value of this technology.</p>
dc.description.comments <p>This article is from <em>Transgenic Research</em> 17 (2008): 59, doi<a href="http://dx.doi.org/10.1007/s11248-007-9081-3" target="_blank">:10.1007/s11248-007-9081-3 </a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/79/
dc.identifier.articleid 1087
dc.identifier.contextkey 8192377
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/79
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/5051
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/79/2008_ScottMP_TransgenicMaizeEndosperm.pdf|||Sat Jan 15 01:55:42 UTC 2022
dc.source.uri 10.1007/s11248-007-9081-3
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Food Science
dc.subject.disciplines Genetics
dc.subject.disciplines Human and Clinical Nutrition
dc.subject.keywords Transgene
dc.subject.keywords Maize
dc.subject.keywords a-Lactalbumin
dc.subject.keywords Lysine
dc.subject.keywords Nutrition
dc.subject.keywords Grain
dc.title Transgenic maize endosperm containing a milk protein has improved amino acid balance
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 97acee5f-1291-4c27-8929-a8e1617c411d
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relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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