A sugarcane mosaic virus vector for gene expression in maize

dc.contributor.author Mei, Yu
dc.contributor.author Liu, Guanjun
dc.contributor.author Whitham, Steven
dc.contributor.author Zhang, Chunquan
dc.contributor.author Hill, John
dc.contributor.author Whitham, Steven
dc.contributor.department Plant Pathology and Microbiology
dc.date 2019-12-16T20:35:23.000
dc.date.accessioned 2020-06-30T06:23:21Z
dc.date.available 2020-06-30T06:23:21Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.issued 2019-08-01
dc.description.abstract <p><em>Zea mays</em> L. ssp. <em>mays</em> (maize) is an important crop plant as well as model system for genetics and plant biology. The ability to select among different virus‐based platforms for transient gene silencing or protein expression experiments is expected to facilitate studies of gene function in maize and complement experiments with stable transgenes. Here, we describe the development of a sugarcane mosaic virus (SCMV) vector for the purpose of protein expression in maize. An infectious SCMV cDNA clone was constructed, and heterologous genetic elements were placed between the protein 1 (P1) and helper component‐proteinase (HC‐Pro) cistrons in the SCMV genome. Recombinant SCMV clones engineered to express green fluorescent protein (GFP), β‐glucuronidase (GUS), or bialaphos resistance (BAR) protein were introduced into sweet corn (Golden × Bantam) plants. Documentation of developmental time courses spanning maize growth from seedling to tasseling showed that the SCMV genome tolerates insertion of foreign sequences of at least 1,809 nucleotides at the P1/HC‐Pro junction. Analysis of insert stability showed that the integrity of GFP and BAR coding sequences was maintained longer than that of the much larger GUS coding sequence. The SCMV isolate from which the expression vector is derived is able to infect several important maize inbred lines, suggesting that this SCMV vector has potential to be a valuable tool for gene functional analysis in a broad range of experimentally important maize genotypes.</p>
dc.description.comments <p>This article is published as Mei, Yu, Guanjun Liu, Chunquan Zhang, John H. Hill, and Steven A. Whitham. "A sugarcane mosaic virus vector for gene expression in maize." <em>Plant Direct</em> 3, no. 8 (2019): e00158. doi: <a href="https://doi.org/10.1002/pld3.158">10.1002/pld3.158</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/265/
dc.identifier.articleid 1266
dc.identifier.contextkey 15122668
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/265
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57717
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/265/2019_Whitham_SugarcaneMosaic.pdf|||Fri Jan 14 23:03:19 UTC 2022
dc.source.uri 10.1002/pld3.158
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.disciplines Plant Pathology
dc.subject.keywords gene expression
dc.subject.keywords green fluorescent protein
dc.subject.keywords maize
dc.subject.keywords monocot
dc.subject.keywords potyvirus
dc.subject.keywords vector
dc.title A sugarcane mosaic virus vector for gene expression in maize
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication bd8b6e79-2efb-4611-9a27-13792f2b9a43
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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