Transcriptome Sequencing Reveals Novel Candidate Genes for Cardinium hertigii-Caused Cytoplasmic Incompatibility and Host-Cell Interaction

Date
2017-01-01
Authors
Mann, Evelyn
Schmitz-Esser, Stephan
Stouthamer, Corinne
Kelly, Suzanne
Dzieciol, Monika
Hunter, Martha
Schmitz-Esser, Stephan
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Animal Science
Organizational Unit
Journal Issue
Series
Abstract

Cytoplasmic incompatibility (CI) is an intriguing, widespread, symbiont-induced reproductive failure that decreases offspring production of arthropods through crossing incompatibility of infected males with uninfected females or with females infected with a distinct symbiont genotype. For years, the molecular mechanism of CI remained unknown. Recent genomic, proteomic, biochemical, and cell biological studies have contributed to understanding of CI in the alphaproteobacterium Wolbachia and implicate genes associated with the WO prophage. Besides a recently discovered additional lineage of alphaproteobacterial symbionts only moderately related to Wolbachia, Cardinium (Bacteroidetes) is the only other symbiont known to cause CI, and genomic evidence suggests that it has very little homology with Wolbachia and evolved this phenotype independently. Here, we present the first transcriptomic study of the CI Cardinium strain cEper1, in its natural host, Encarsia suzannae, to detect important CI candidates and genes involved in the insect-Cardinium symbiosis. Highly expressed transcripts included genes involved in manipulating ubiquitination, apoptosis, and host DNA. Female-biased genes encoding ribosomal proteins suggest an increase in general translational activity of Cardinium in female wasps. The results confirm previous genomic analyses that indicated that Wolbachia and Cardinium utilize different genes to induce CI, and transcriptome patterns further highlight expression of some common pathways that these bacteria use to interact with the host and potentially cause this enigmatic and fundamental manipulation of host reproduction.

Description
<p>This article is published as Mann, Evelyne, Corinne M. Stouthamer, Suzanne E. Kelly, Monika Dzieciol, Martha S. Hunter, and Stephan Schmitz-Esser. "Transcriptome sequencing reveals novel candidate genes for Cardinium hertigii-caused cytoplasmic incompatibility and host-cell interaction." <em>MSystems</em> 2, no. 6 (2017): e00141-17. doi: <a href="http://dx.doi.org/10.1128/mSystems.00141-17" target="_blank">10.1128/mSystems.00141-17</a>.</p>
Keywords
Bacteroidetes, Cardinium, cytoplasmic incompatibility, host-microbe interaction, RNA sequencing, endosymbionts, gene expression
Citation
Collections