Transcriptional defense response of the soybean plant, Glycine max, in reaction to infestation by the soybean aphid, Aphis glycines

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2010-01-01
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Studham, Matthew
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Gustavo C. Macintosh
Julie A. Dickerson
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Biochemistry, Biophysics and Molecular Biology

The Department of Biochemistry, Biophysics, and Molecular Biology was founded to give students an understanding of life principles through the understanding of chemical and physical principles. Among these principles are frontiers of biotechnology such as metabolic networking, the structure of hormones and proteins, genomics, and the like.

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The Department of Biochemistry and Biophysics was founded in 1959, and was administered by the College of Sciences and Humanities (later, College of Liberal Arts & Sciences). In 1979 it became co-administered by the Department of Agriculture (later, College of Agriculture and Life Sciences). In 1998 its name changed to the Department of Biochemistry, Biophysics, and Molecular Biology.

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1959–present

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  • Department of Biochemistry and Biophysics (1959–1998)

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Abstract

The soybean aphid (Aphis glycines), first spotted in North American fields in 2000, is a major pest of the soybean plant (Glycine max) and can cause millions of dollars in yield losses. The goal of my project was to reveal the transcriptional defense response to soybean aphids in aphid-susceptible soybean plants and aphid-resistant soybean plants with the Rag1 gene. These responses were determined by using Affymetrix GeneChip® Soybean Genome Arrays to characterize transcript levels from leaf tissue collected after one, seven, and twenty-one days of aphid infestation. In addition to the microarray analysis, a bioinformatics tool was developed to determine the relative induction of the plant hormones abscisic acid, ethylene, jasmonic acid, and salicylic acid based on the transcript levels. My analysis, in conjunction with prior research, led to conclusions regarding the soybean plant's defense against aphids. These include the identification of an effective defense hormone, defense compounds, and possible decoy defense strategies employed by the soybean aphid.

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Fri Jan 01 00:00:00 UTC 2010