Sustained antigen release polyanhydride-based vaccine platform for immunization against bovine brucellosis

dc.contributor.author Boggiatto, Paola
dc.contributor.author Schaut, Robert
dc.contributor.author Kanipe, Carly
dc.contributor.author Kelly, Sean
dc.contributor.author Narasimhan, Balaji
dc.contributor.author Narasimhan, Balaji
dc.contributor.author Jones, Douglas
dc.contributor.author Olsen, Steven
dc.contributor.department Veterinary Pathology
dc.contributor.department Chemical and Biological Engineering
dc.contributor.department Nanovaccine Institute
dc.date 2019-09-22T06:11:53.000
dc.date.accessioned 2020-06-30T01:10:09Z
dc.date.available 2020-06-30T01:10:09Z
dc.date.issued 2019-08-01
dc.description.abstract <p>Brucellosis is a bacterial zoonosis and a significant source of economic loss and a major public health concern, worldwide. Bovine brucellosis, as caused primarily by Brucella abortus, is an important cause of reproductive loss in cattle. Vaccination has been the most effective way to reduce disease prevalence contributing to the success of control and eradication programs. Currently, there are no human vaccines available, and despite the success of commercial vaccines for livestock, such as B. abortus strain RB51 (RB51), there is need for development of novel and safer vaccines against brucellosis. In the current study, we report the fabrication of and immune responses to an implantable single dose polyanhydride-based, methanol-killed RB51 antigen containing delivery platform (VPEAR) in cattle. In contrast to animals vaccinated with RB51, we did not observe measurable RB51-specific IFN-γ or IgG responses in the peripheral blood, following initial vaccination with VPEAR. However, following a subsequent booster vaccination with RB51, we observed an anamnestic response in both vaccination treatments (VPEAR and live RB51). The magnitude and kinetics of CD4+ IFN-γ-mediated responses and circulating memory T cell subpopulations were comparable between the two vaccination treatments. Additionally, IgG titers were significantly increased in animals vaccinated with VPEAR as compared to live RB51- vaccinated animals. These data demonstrate that killed antigen may be utilized to generate and sustain memory, IFN-γ-mediated, CD4+ T cell and humoral responses against Brucella in a natural host. To our knowledge, this novel approach to vaccination against intracellular bacteria, such as Brucella, has not been reported before.</p>
dc.description.comments <p>This article is published as Boggiatto, Paola M., Robert G. Schaut, Carly Kanipe, Sean M. Kelly, Balaji Narasimhan, Douglas E. Jones, and Steven C. Olsen. "Sustained antigen release polyanhydride-based vaccine platform for immunization against bovine brucellosis." <em>Heliyon</em> 5, no. 8 (2019): e02370. DOI: <a href="http://dx.doi.org/10.1016/j.heliyon.2019.e02370" target="_blank">10.1016/j.heliyon.2019.e02370</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/388/
dc.identifier.articleid 1389
dc.identifier.contextkey 15325739
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/388
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13491
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/388/2019_NarasimhanBalaji_SustainedAntigen.pdf|||Fri Jan 14 23:54:03 UTC 2022
dc.source.uri 10.1016/j.heliyon.2019.e02370
dc.subject.disciplines Immunology of Infectious Disease
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Immunology
dc.subject.keywords Infectious disease
dc.subject.keywords Vaccines
dc.subject.keywords Immune response
dc.subject.keywords Vaccination
dc.subject.keywords Antibody
dc.subject.keywords Bacteria
dc.subject.keywords Polyanhydride
dc.subject.keywords Vaccine
dc.subject.keywords Cattle
dc.subject.keywords Single-dose
dc.subject.keywords Brucella
dc.title Sustained antigen release polyanhydride-based vaccine platform for immunization against bovine brucellosis
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 185bb428-638b-41cf-91ff-d997d87c3543
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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