Characterization of RNS2, an S-Like RNase in Arabidopsis thaliana suggests role in phosphate recycling

dc.contributor.advisor Gustavo MacIntosh
dc.contributor.author Ohanian, Saralyn
dc.contributor.department Theses & dissertations (Interdisciplinary)
dc.date 2018-08-23T05:24:12.000
dc.date.accessioned 2020-06-30T07:41:36Z
dc.date.available 2020-06-30T07:41:36Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.issued 2007-01-01
dc.description.abstract <p>Ribonucleases play important biological roles within all organisms but many have not been well characterized. The family of T2 RNases is well-conserved throughout the kingdoms of life and yet their properties and functions are not well understood. RNS2 is one of five such RNases found in Arabidopsis, and preliminary evidence shows that it may be involved in phosphate recycling and localized to an intracellular compartment. Using rns2 knock-out mutant plants, I showed that RNS2 has an effect on flowering time during times of phosphate starvation. I also showed that RNS2 is localized to the ER, more specifically, to ER-bodies. To explore the hypothesis that a homologous gene from C. elegans (CeRNS) plays a role in RNAi in nematodes I used RNAi to reduce CeRNS expression, but I did not observe obvious phenotype abnormalities. Additionally, I did not observe consistent effects of CeRNS RNAi on C. elegans' response to RNAi.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/15066/
dc.identifier.articleid 16065
dc.identifier.contextkey 7015071
dc.identifier.doi https://doi.org/10.31274/rtd-180813-16200
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/15066
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/68660
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/15066/1446065.PDF|||Fri Jan 14 20:35:11 UTC 2022
dc.subject.disciplines Molecular Biology
dc.subject.keywords Biochemistry
dc.subject.keywords biophysics
dc.subject.keywords and molecular biology;Molecular
dc.subject.keywords cellular
dc.subject.keywords and developmental biology;
dc.title Characterization of RNS2, an S-Like RNase in Arabidopsis thaliana suggests role in phosphate recycling
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
thesis.degree.discipline Molecular, Cellular, and Developmental Biology
thesis.degree.level thesis
thesis.degree.name Master of Science
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