The Linker between SH2 and Kinase Domains Positively Regulates Catalysis of the Tec Family Kinases

dc.contributor.author Joseph, Raji
dc.contributor.author Min, Lie
dc.contributor.author Andreotti, Amy
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2018-02-17T03:47:32.000
dc.date.accessioned 2020-06-29T23:46:09Z
dc.date.available 2020-06-29T23:46:09Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.issued 2007-04-01
dc.description.abstract <p>Tec family nonreceptor tyrosine kinases are key immunological enzymes that control processes that range from T and B cell development to reorganization of the actin cytoskeleton. The full-length Tec kinases have been resistant to crystallization. This lack of structural data and the paucity of <em>in vitro</em> biochemical data for this kinase family leave a void in our understanding of Tec kinase regulation. In this report we have used interleukin-2 tyrosine kinase (Itk) as a model system to gain insight into the regulatory apparatus of the Tec kinases. Use of a quantitative <em>in vitro</em> kinase assay has uncovered an essential role for the short linker region flanked by the SH2 and kinase domains of Itk in positively regulating Itk catalytic activity. The precise residues that allosterically regulate Itk are conserved among Tec kinases, pointing to the conserved nature of this regulatory mechanism within the family. These findings indicate that Tec kinases are not regulated in the same manner as the Src kinases but rather share some of the regulatory features of Csk instead.</p>
dc.description.comments <p>Reprinted (adapted) with permission from <em>Biotechnology</em> 46 (2007): 5455, doi:<a href="http://dx.doi.org/10.1021/bi602512e" target="_blank">10.1021/bi602512e</a>. Copyright 2007 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/17/
dc.identifier.articleid 1028
dc.identifier.contextkey 7747773
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/17
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/10632
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/17/0-AndreottiLicense_LinkerBetween.pdf|||Fri Jan 14 21:11:05 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/17/2007_Andreotti_LinkerBetweenSH2.pdf|||Fri Jan 14 21:11:07 UTC 2022
dc.source.uri 10.1021/bi602512e
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Molecular Biology
dc.title The Linker between SH2 and Kinase Domains Positively Regulates Catalysis of the Tec Family Kinases
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 97143b55-8496-4365-8699-e254a8ac0903
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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