Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode Meloidogyne incognita and Expression Analysis During Plant Parasitism

Date
1999-07-01
Authors
Rosso, Marie-Noëlle
Favery, Bruno
Piotte, Christine
Arthaud, Laury
De Boer, Jan
Hussey, Richard
Bakker, Japp
Baum, Thomas
Abad, Pierre
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A β-1,4-endoglucanase encoding cDNA (EGases, E.C. 3.2.1.4), named Mi-eng-1, was cloned from Meloidogyne incognita second-stage juveniles (J2). The deduced amino acid sequence contains a catalytic domain and a cellulose-binding domain separated by a linker. In M. incognita, the gene is transcribed in the migratory J2, in males, and in the sedentary adult females. In pre-parasitic J2, endoglucanase transcripts are located in the cytoplasm of the subventral esophageal glands. The presence of β-1,4-endoglucanase transcripts in adult females could be related to the expression of the gene in esophageal glands at this stage. However, cellulase activity within the egg matrix of adult females suggests that the endoglucanase may also be synthesized in the rectal glands and involved in the extrusion of the eggs onto the root surface. The maximum identity of the predicted MI-ENG-1 catalytic domain with the recently cloned cyst nematode β-1,4-endoglucanases is 52.5%. In contrast to cyst nematodes, M. incognita pre-parasitic J2 were not found to express a β-1,4-endoglucanase devoid of a cellulose-binding domain.

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This article is published as Rosso, Marie-Noëlle, Bruno Favery, Christine Piotte, Laury Arthaud, Jan M. De Boer, Richard S. Hussey, Jaap Bakker, Thomas J. Baum, and Pierre Abad. "Isolation of a cDNA encoding a β-1, 4-endoglucanase in the root-knot nematode Meloidogyne incognita and expression analysis during plant parasitism." Molecular Plant-Microbe Interactions 12, no. 7 (1999): 585-591, doi: 10.1094/MPMI.1999.12.7.585.

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