Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode Meloidogyne incognita and Expression Analysis During Plant Parasitism Rosso, Marie-Noëlle Baum, Thomas Favery, Bruno Piotte, Christine Arthaud, Laury De Boer, Jan Hussey, Richard Bakker, Japp Baum, Thomas Abad, Pierre
dc.contributor.department Plant Pathology and Microbiology 2018-02-18T18:48:08.000 2020-06-30T06:22:28Z 2020-06-30T06:22:28Z 1999-07-01
dc.description.abstract <p>A β-1,4-endoglucanase encoding cDNA (EGases, E.C., named <em>Mi-eng-1</em>, was cloned from <em>Meloidogyne incognita</em> second-stage juveniles (J2). The deduced amino acid sequence contains a catalytic domain and a cellulose-binding domain separated by a linker. In <em>M. incognita</em>, the gene is transcribed in the migratory J2, in males, and in the sedentary adult females. In pre-parasitic J2, endoglucanase transcripts are located in the cytoplasm of the subventral esophageal glands. The presence of β-1,4-endoglucanase transcripts in adult females could be related to the expression of the gene in esophageal glands at this stage. However, cellulase activity within the egg matrix of adult females suggests that the endoglucanase may also be synthesized in the rectal glands and involved in the extrusion of the eggs onto the root surface. The maximum identity of the predicted MI-ENG-1 catalytic domain with the recently cloned cyst nematode β-1,4-endoglucanases is 52.5%. In contrast to cyst nematodes, <em>M. incognita</em> pre-parasitic J2 were not found to express a β-1,4-endoglucanase devoid of a cellulose-binding domain.</p>
dc.description.comments <p>This article is published as Rosso, Marie-Noëlle, Bruno Favery, Christine Piotte, Laury Arthaud, Jan M. De Boer, Richard S. Hussey, Jaap Bakker, Thomas J. Baum, and Pierre Abad. "Isolation of a cDNA encoding a β-1, 4-endoglucanase in the root-knot nematode Meloidogyne incognita and expression analysis during plant parasitism." <em>Molecular Plant-Microbe Interactions</em> 12, no. 7 (1999): 585-591, doi: <a href="">10.1094/MPMI.1999.12.7.585</a>. </p>
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dc.identifier.articleid 1169
dc.identifier.contextkey 10538534
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dc.source.uri 10.1094/MPMI.1999.12.7.585
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Pathology
dc.title Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode Meloidogyne incognita and Expression Analysis During Plant Parasitism
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