Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode Meloidogyne incognita and Expression Analysis During Plant Parasitism

dc.contributor.author Rosso, Marie-Noëlle
dc.contributor.author Baum, Thomas
dc.contributor.author Favery, Bruno
dc.contributor.author Piotte, Christine
dc.contributor.author Arthaud, Laury
dc.contributor.author De Boer, Jan
dc.contributor.author Hussey, Richard
dc.contributor.author Bakker, Japp
dc.contributor.author Baum, Thomas
dc.contributor.author Abad, Pierre
dc.contributor.department Plant Pathology and Microbiology
dc.date 2018-02-18T18:48:08.000
dc.date.accessioned 2020-06-30T06:22:28Z
dc.date.available 2020-06-30T06:22:28Z
dc.date.issued 1999-07-01
dc.description.abstract <p>A β-1,4-endoglucanase encoding cDNA (EGases, E.C. 3.2.1.4), named <em>Mi-eng-1</em>, was cloned from <em>Meloidogyne incognita</em> second-stage juveniles (J2). The deduced amino acid sequence contains a catalytic domain and a cellulose-binding domain separated by a linker. In <em>M. incognita</em>, the gene is transcribed in the migratory J2, in males, and in the sedentary adult females. In pre-parasitic J2, endoglucanase transcripts are located in the cytoplasm of the subventral esophageal glands. The presence of β-1,4-endoglucanase transcripts in adult females could be related to the expression of the gene in esophageal glands at this stage. However, cellulase activity within the egg matrix of adult females suggests that the endoglucanase may also be synthesized in the rectal glands and involved in the extrusion of the eggs onto the root surface. The maximum identity of the predicted MI-ENG-1 catalytic domain with the recently cloned cyst nematode β-1,4-endoglucanases is 52.5%. In contrast to cyst nematodes, <em>M. incognita</em> pre-parasitic J2 were not found to express a β-1,4-endoglucanase devoid of a cellulose-binding domain.</p>
dc.description.comments <p>This article is published as Rosso, Marie-Noëlle, Bruno Favery, Christine Piotte, Laury Arthaud, Jan M. De Boer, Richard S. Hussey, Jaap Bakker, Thomas J. Baum, and Pierre Abad. "Isolation of a cDNA encoding a β-1, 4-endoglucanase in the root-knot nematode Meloidogyne incognita and expression analysis during plant parasitism." <em>Molecular Plant-Microbe Interactions</em> 12, no. 7 (1999): 585-591, doi: <a href="https://doi.org/10.1094/MPMI.1999.12.7.585">10.1094/MPMI.1999.12.7.585</a>. </p>
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dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/156/
dc.identifier.articleid 1169
dc.identifier.contextkey 10538534
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/156
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57599
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/156/1999_Baum_IsolationEncoding.pdf|||Fri Jan 14 20:43:40 UTC 2022
dc.source.uri 10.1094/MPMI.1999.12.7.585
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Pathology
dc.title Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode Meloidogyne incognita and Expression Analysis During Plant Parasitism
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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