Multilocus Sequence Typing for Characterization of Non typhoidal Salmonella Strains in Swine Isolated on Farm and at Slaughter
Is Version Of
The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.
Nontyphoidal Salmonella arc among the leading causes of food-borne disease in the United States. The aim of this study was to compare nontyphoidal Salmonella spatially and temporally in pig production and design a source tracking tool for Salmonella shedding on farm and contamination at slaughter. In this study, a total of265 nontyphoidal Salmonella spp. were isolated from 20 Iowa herds which were enrolled by cooperative agreement and visited from September 2006 to February 2009. Eleven of the 20 herds had multiple visits. At each farm visit, 30 individual fecal samples were collected. At slaughter, 30 mesenteric lymph nodes were collected. Samples were tested for Salmonella spp. using conventional microbiological methods and serotyped at the National Veterinary Services Laboratory (NVSL). All the 265 Salmonella isolates were further characterized by multilocus sequence typing (MLST).