Genetic characterization of maize populations selected for grain methionine content

dc.contributor.advisor M. P. Scott
dc.contributor.author Vogel, Karla
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-08-11T08:08:57.000
dc.date.accessioned 2020-06-30T02:37:49Z
dc.date.available 2020-06-30T02:37:49Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2010
dc.date.embargo 2013-06-05
dc.date.issued 2010-01-01
dc.description.abstract <p>Methionine is one of three essential amino acids that are nutritionally limiting in maize, which is a major component of animal diets. Diets consisting mainly of maize must be supplemented with other sources of methionine to provide adequate nutrition. Plant breeding strategies have been implemented to increase the level of methionine in maize. The objective of this study was to genetically characterize two programs involving recurrent selection for grain methionine content. These programs started with the synthetic populations BS11 or BS31. We sought to determine the genetic effects this selection process has had on the genome. Single nucleotide polymorphism (SNP) markers distributed across the genome and in gene regions potentially related to methionine levels were examined using allele frequency based and linkage disequilibrium (LD) analyses. The percentage of polymorphic loci decreased through the cycles of selection in both populations and alleles present in the starting populations were lost. The two measures of LD used in this study, D' and r2, demonstrate an increase in disequilibrium across the genome for both BS11- and BS31- derived populations suggesting selection occurred at multiple loci. Several genetic loci exhibited allele frequency changes that paralleled the advancement of the selection program.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/11738/
dc.identifier.articleid 2690
dc.identifier.contextkey 2807888
dc.identifier.doi https://doi.org/10.31274/etd-180810-2351
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/11738
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/25944
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/11738/Vogel_iastate_0097M_11550.pdf|||Fri Jan 14 18:57:18 UTC 2022
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.keywords maize
dc.subject.keywords methionine
dc.subject.keywords molecular markers
dc.subject.keywords recurrent selection
dc.title Genetic characterization of maize populations selected for grain methionine content
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
thesis.degree.level thesis
thesis.degree.name Master of Science
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